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Literature summary extracted from

  • Sawaya, R.; Schwer, B.; Shuman, S.
    Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1 (2005), RNA, 11, 107-113.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.160 expression from Saccharomyces cerevisiae vectors Saccharomyces cerevisiae
2.7.1.160 expression in Saccharomyces cerevisiae Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.160 F72A temperature sensitive Saccharomyces cerevisiae
2.7.1.160 H117A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 H117A-T119A lethal in vivo Saccharomyces cerevisiae
2.7.1.160 H142A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 H22A lethal in vivo Escherichia coli
2.7.1.160 H24A lethal in vivo Saccharomyces cerevisiae
2.7.1.160 H24N lethal in vivo Saccharomyces cerevisiae
2.7.1.160 H24Q lethal in vivo Saccharomyces cerevisiae
2.7.1.160 H90A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 K16A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 K69A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 R125A lethal in vivo Escherichia coli
2.7.1.160 R138A lethal in vivo Saccharomyces cerevisiae
2.7.1.160 R138K colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 R138Q lethal in vivo Saccharomyces cerevisiae
2.7.1.160 R158A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 R21A lethal in vivo Escherichia coli
2.7.1.160 R23A lethal in vivo Saccharomyces cerevisiae
2.7.1.160 R23K lethal in vivo Saccharomyces cerevisiae
2.7.1.160 R23Q lethal in vivo Saccharomyces cerevisiae
2.7.1.160 R69A lethal in vivo Escherichia coli
2.7.1.160 R71A lethal in vivo Saccharomyces cerevisiae
2.7.1.160 R71K lethal in vivo Saccharomyces cerevisiae
2.7.1.160 R71Q grows slower than wild-type, temperature sensitive Saccharomyces cerevisiae
2.7.1.160 S91A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 T119A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
2.7.1.160 Y38A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.160 25000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.160 Aeropyrum pernix
-
-
-
2.7.1.160 Archaeoglobus fulgidus
-
-
-
2.7.1.160 Clostridium perfringens
-
-
-
2.7.1.160 Drosophila melanogaster
-
-
-
2.7.1.160 Escherichia coli
-
-
-
2.7.1.160 Homo sapiens
-
-
-
2.7.1.160 Leishmania major
-
-
-
2.7.1.160 Nostoc punctiforme
-
-
-
2.7.1.160 Pyrococcus horikoshii
-
-
-
2.7.1.160 Saccharomyces cerevisiae
-
-
-
2.7.1.160 Schizosaccharomyces pombe
-
-
-
2.7.1.160 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.160 Ni-agarose chromatography Escherichia coli
2.7.1.160 Ni-agarose chromatography Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.160 -80, Tris-HCl buffer, pH 8.0, 10% glycerol Saccharomyces cerevisiae

Subunits

EC Number Subunits Comment Organism
2.7.1.160 monomer SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.7.1.160 KptA
-
 Escherichia coli
2.7.1.160 Tpt1
-
 Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.160 18 37 growth of mutants K16A, K69A, H90A, S91A, H117A, T119A, H142A, R158A and R138K on yeast medium/peptone/dextrose agar, colony sizes were similar to that of wild-type TPT1 cells Saccharomyces cerevisiae
2.7.1.160 18 30 F27A, R71Q, TPT1(1-220) and TPT1(1-210) cells failed to grow at 37°C on yeast medium/peptone/dextrose agar, R71Q and TPT1(1-210) strains grew slower than wild-type TPT1 Saccharomyces cerevisiae
2.7.1.160 25 30 growth of mutants K16A, Y38A, K69A, F72A, H90A, S91A, H117A, T119A, H142A, R158A, TPT1(1-220) and TPT1(1-210) on 5-fluoroorotic acid agar medium Saccharomyces cerevisiae