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Literature summary extracted from

  • Tyagi, R.; Duquerroy, S.; Navaza, J.; Guddat, L.W.; Duggleby, R.G.
    The crystal structure of a bacterial class II ketol-acid reductoisomerase: domain conservation and evolution (2005), Protein Sci., 14, 3089-3100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.86 expression as His6-tagged enzyme Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.86 purified recombinant His-tagged enzyme, 9 mg/ml protein in 20 mM sodium HEPES, pH 7.5, and NADPH in a ratio of 10 mol NADPH per mol of enzyme, hanging drop vapour diffusion method, equal volumes of 0.003 ml of protein and reservoir solution, the latter containing 1.6 M ammonium sulfate, and 0.1 M sodium bicine, pH 9.0, 17°C, 6 months, X-ray diffraction structure determination and analysis at 2.6 A resolution Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86 recombinant His6-tagged enzyme Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.86 (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ active site structure Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ the enzyme is involved in biosynthesis of the branched chain amino acids valine and leucine, pathway overview Escherichia coli (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.86 tetramer three-dimensional enzyme structure, subunit domain structures, surface contact and interlock, crystal structure analysis, overview Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 class II ketol-acid reductoisomerase
-
 Escherichia coli
1.1.1.86 KARI
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADP+
-
 Escherichia coli