Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Garg, S.K.; Suhail Alam, M.; Soni, V.; Radha Kishan, K.V.; Agrawal, P.
    Characterization of Mycobacterium tuberculosis WhiB1/Rv3219 as a protein disulfide reductase (2007), Protein Expr. Purif., 52, 422-432.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.8 expressed in Escherichia coli Mycobacterium tuberculosis
1.8.4.2 expressed in Escherichia coli strain D5alpha Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.8 14000
-
 2 * 14000, SDS-PAGE Mycobacterium tuberculosis
1.8.1.8 29000
-
 gel filtration Mycobacterium tuberculosis
1.8.4.2 14000
-
 2 * 14000, SDS-PAGE Mycobacterium tuberculosis
1.8.4.2 14056
-
 2 * 14056, unmodified recombinant WhiB1, MALDI-TOF mass spectrometry Mycobacterium tuberculosis
1.8.4.2 14284
-
 2 * 14284, reduced protein after alkylation corresponding to the theoretical mass of the recombinant protein with four iodoacetamide mediated modifications of cysteines, MALDI-TOF mass spectrometry Mycobacterium tuberculosis
1.8.4.2 29000
-
 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.8 Mycobacterium tuberculosis
-
-
-
1.8.1.8 Mycobacterium tuberculosis Rv3219
-
-
-
1.8.4.2 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.8 Ni2+-NTA affinity chromatography Mycobacterium tuberculosis
1.8.4.2 Ni2+-NTA affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.8 additional information
-
 cell extract: 1.56 U/mg, after purification: 8.17 U/mg, one unit is defined as the amount of protein that produces an increase in 0.1 OD in 1 min at 650 nm Mycobacterium tuberculosis
1.8.4.2 additional information
-
 1.56 U/mg in the cell extract, 8.17 U/mg after Ni2+-NTA chromatography purification, one unit is defined as the amount of protein that produces an increase in 0.1 OD in 1 min at 650 nm Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.8 insulin disulfide + ?
-
 Mycobacterium tuberculosis ?
-
 ?
1.8.1.8 insulin disulfide + ?
-
 Mycobacterium tuberculosis Rv3219 ?
-
 ?
1.8.4.2 GSH + protein disulfide
-
 Mycobacterium tuberculosis GSSG + protein-dithiol
-
 ?
1.8.4.2 GSH + protein disulfide
-
 Mycobacterium tuberculosis Rv3219 GSSG + protein-dithiol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.8 homodimer 2 * 14000, SDS-PAGE Mycobacterium tuberculosis
1.8.4.2 homodimer 2 * 14000, SDS-PAGE Mycobacterium tuberculosis
1.8.4.2 homodimer 2 * 14056, unmodified recombinant WhiB1, MALDI-TOF mass spectrometry Mycobacterium tuberculosis
1.8.4.2 homodimer 2 * 14284, reduced protein after alkylation corresponding to the theoretical mass of the recombinant protein with four iodoacetamide mediated modifications of cysteines, MALDI-TOF mass spectrometry Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.8.1.8 protein disulfide reductase
-
 Mycobacterium tuberculosis
1.8.1.8 WhiB1 wild type enzyme Mycobacterium tuberculosis
1.8.1.8 WhiB1/Rv3219 recombinant enzyme Mycobacterium tuberculosis
1.8.4.2 WhiB1
-
 Mycobacterium tuberculosis
1.8.4.2 WhiB1/Rv3219
-
 Mycobacterium tuberculosis