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Literature summary extracted from

  • Kaiser, P.; Raina, C.; Parshad, R.; Johri, S.; Verma, V.; Andrabi, K.I.; Qazi, G.N.
    A novel esterase from Bacillus subtilis (RRL 1789): purification and characterization of the enzyme (2006), Protein Expr. Purif., 45, 262-268.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Ag+ 1 mM, complete inhibition Bacillus subtilis
3.1.1.1 Fe3+ partial Bacillus subtilis
3.1.1.1 Hg2+ 1 mM, complete inhibition Bacillus subtilis
3.1.1.1 Mn2+ partial Bacillus subtilis
3.1.1.1 additional information not inhibitory: EDTA, EGTA, Li+, K+, NH4+, Mg2+, Triton X-100 at 0.1%, dimethylsulfoxide at 15% Bacillus subtilis
3.1.1.1 phenylmethylsulfonyl fluoride 1 mM, complete inhibition Bacillus subtilis
3.1.1.1 sodium dodecylsulfate 0.1% w/V, 10% residual activity Bacillus subtilis
3.1.1.1 Zn2+ 1 mM, complete inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.004
-
 4-nitrophenyl butanoate 37°C Bacillus subtilis
3.1.1.1 0.008
-
 4-nitrophenyl caproate 37°C Bacillus subtilis
3.1.1.1 0.017
-
 4-nitrophenyl caprylate 37°C Bacillus subtilis
3.1.1.1 0.05
-
 4-nitrophenyl acetate 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 Ca2+ about 20% stimulation Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 52000
-
 x * 52000, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Bacillus subtilis
-
 strasin RRL 1789
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 944
-
 37°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Bacillus subtilis 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Bacillus subtilis 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O
-
 Bacillus subtilis 4-nitrophenol + caproate
-
 ?
3.1.1.1 4-nitrophenyl caprylate + H2O
-
 Bacillus subtilis 4-nitrophenol + caprylate
-
 ?
3.1.1.1 triacetin + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.1.1 tributyrin + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.1.1 tricaproin + H2O poor substrate Bacillus subtilis ?
-
 ?
3.1.1.1 tricaprylin + H2O poor substrate Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 52000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 BSE
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 37
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 55
-
 1 h, 50% residual activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 6 9
-
 Bacillus subtilis