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Literature summary extracted from

  • Levasseur, A.; Benoit, I.; Asther, M.; Asther, M.; Record, E.
    Homologous expression of the feruloyl esterase B gene from Aspergillus niger and characterization of the recombinant enzyme (2004), Protein Expr. Purif., 37, 126-133.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 nutrition broad range of application in the agri-food industries, production of ferulic as food preservative and as potential precursor for the production of flavor and photo-active agents Aspergillus niger
3.1.1.73 paper production potential use in pulp bleaching Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expressed as His-tag fusion protein in a protease deficient Aspergillus niger strain Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.03
-
 methyl p-coumarate
-
 Aspergillus niger
3.1.1.73 0.13
-
 methyl caffeate
-
 Aspergillus niger
3.1.1.73 0.16
-
 5-O-caffeoyl quinic acid
-
 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 75000
-
 2 * 75000, SDS-PAGE, native mass by gel filtration Aspergillus niger
3.1.1.73 150000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O Aspergillus niger involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O Aspergillus niger BRFM131 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger Q8WZI8 feruloyl esterase B
-
3.1.1.73 Aspergillus niger BRFM131 Q8WZI8 feruloyl esterase B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinant enzyme using His-tag Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 5-O-caffeoyl quinic acid + H2O
-
 Aspergillus niger ?
-
 ?
3.1.1.73 5-O-caffeoyl quinic acid + H2O
-
 Aspergillus niger BRFM131 ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus niger ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus niger BRFM131 ferulic acid + ?
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus niger caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus niger BRFM131 caffeic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus niger p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus niger BRFM131 p-coumaric acid + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 dimer 2 * 75000, SDS-PAGE, native mass by gel filtration Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEB
-
 Aspergillus niger
3.1.1.73 feruloyl esterase B
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 40
-
 stable Aspergillus niger
3.1.1.73 45 50 10-16% loss of activity within 120 min Aspergillus niger
3.1.1.73 55
-
 half life: 30 min Aspergillus niger
3.1.1.73 60
-
 rapid inactivation Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6
-
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Aspergillus niger isoelectric focusing
-
 4.8