EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.73 | nutrition | broad range of application in the agri-food industries, production of ferulic as food preservative and as potential precursor for the production of flavor and photo-active agents | Aspergillus niger |
3.1.1.73 | paper production | potential use in pulp bleaching | Aspergillus niger |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.73 | expressed as His-tag fusion protein in a protease deficient Aspergillus niger strain | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.03 | - |
methyl p-coumarate | - |
Aspergillus niger | |
3.1.1.73 | 0.13 | - |
methyl caffeate | - |
Aspergillus niger | |
3.1.1.73 | 0.16 | - |
5-O-caffeoyl quinic acid | - |
Aspergillus niger |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.73 | extracellular | - |
Aspergillus niger | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 75000 | - |
2 * 75000, SDS-PAGE, native mass by gel filtration | Aspergillus niger |
3.1.1.73 | 150000 | - |
gel filtration | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | feruloyl polysaccharide + H2O | Aspergillus niger | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
3.1.1.73 | feruloyl polysaccharide + H2O | Aspergillus niger BRFM131 | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Aspergillus niger | Q8WZI8 | feruloyl esterase B | - |
3.1.1.73 | Aspergillus niger BRFM131 | Q8WZI8 | feruloyl esterase B | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | recombinant enzyme using His-tag | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | 5-O-caffeoyl quinic acid + H2O | - |
Aspergillus niger | ? | - |
? | |
3.1.1.73 | 5-O-caffeoyl quinic acid + H2O | - |
Aspergillus niger BRFM131 | ? | - |
? | |
3.1.1.73 | feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Aspergillus niger | ferulic acid + ? | - |
? | |
3.1.1.73 | feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Aspergillus niger BRFM131 | ferulic acid + ? | - |
? | |
3.1.1.73 | methyl caffeate + H2O | - |
Aspergillus niger | caffeic acid + methanol | - |
? | |
3.1.1.73 | methyl caffeate + H2O | - |
Aspergillus niger BRFM131 | caffeic acid + methanol | - |
? | |
3.1.1.73 | methyl p-coumarate + H2O | - |
Aspergillus niger | p-coumaric acid + methanol | - |
? | |
3.1.1.73 | methyl p-coumarate + H2O | - |
Aspergillus niger BRFM131 | p-coumaric acid + methanol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | dimer | 2 * 75000, SDS-PAGE, native mass by gel filtration | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | FAEB | - |
Aspergillus niger |
3.1.1.73 | feruloyl esterase B | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 50 | - |
- |
Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 40 | - |
stable | Aspergillus niger |
3.1.1.73 | 45 | 50 | 10-16% loss of activity within 120 min | Aspergillus niger |
3.1.1.73 | 55 | - |
half life: 30 min | Aspergillus niger |
3.1.1.73 | 60 | - |
rapid inactivation | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | - |
- |
Aspergillus niger |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.73 | Aspergillus niger | isoelectric focusing | - |
4.8 |