EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.32 | EDTA | 23.9% activation at 10 mM | Campylobacter jejuni |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.32 | gene hipO, expression of His6-tagged enzyme in Escherichia coli strain M15 | Campylobacter jejuni |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.32 | D104N | site-directed mutagenesis, inactive mutant | Campylobacter jejuni |
3.5.1.32 | D76N | site-directed mutagenesis, the mutant shows over 12fold reduced activity compared to the wild-type enzyme | Campylobacter jejuni |
3.5.1.32 | E134Q | site-directed mutagenesis, inactive mutant | Campylobacter jejuni |
3.5.1.32 | E135Q | site-directed mutagenesis, inactive mutant | Campylobacter jejuni |
3.5.1.32 | H161A | site-directed mutagenesis, inactive mutant | Campylobacter jejuni |
3.5.1.32 | H356A | site-directed mutagenesis, inactive mutant | Campylobacter jejuni |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.32 | 1,10-phenanthroline | 99% inhibition at 0.5 mM | Campylobacter jejuni | |
3.5.1.32 | 2-mercaptoethanol | 20% inhibition at 2 mM | Campylobacter jejuni | |
3.5.1.32 | Ag+ | 98% inhibition at 2 mM | Campylobacter jejuni | |
3.5.1.32 | Ca2+ | 55% inhibition at 2 mM | Campylobacter jejuni | |
3.5.1.32 | Cd2+ | 65% inhibition at 2 mM | Campylobacter jejuni | |
3.5.1.32 | Co2+ | 70% inhibition at 2 mM | Campylobacter jejuni | |
3.5.1.32 | Cu2+ | 78% inhibition at 2 mM | Campylobacter jejuni | |
3.5.1.32 | Fe2+ | - |
Campylobacter jejuni | |
3.5.1.32 | additional information | no significant inhibition by E64, PMSF, and pepstatin at 0.5 mM | Campylobacter jejuni | |
3.5.1.32 | Ni2+ | 65% inhibition at 2 mM | Campylobacter jejuni | |
3.5.1.32 | Zn2+ | 35% inhibition at 2 mM | Campylobacter jejuni |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.32 | 0.159 | - |
N-benzoylglutamate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 0.169 | - |
N-benzoylalanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 0.195 | - |
N-benzoylphenylalanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 0.303 | - |
N-benzoylmethionine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 0.594 | - |
N-benzoylvaline | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 0.65 | - |
hippurate | pH 7.5, 37°C, native enzyme in cell extract | Campylobacter jejuni | |
3.5.1.32 | 0.764 | - |
hippurate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 1.769 | - |
4-aminobenzoyl-glutamate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 2.778 | - |
hippurate | pH 7.5, 37°C, recombinant mutant D76N | Campylobacter jejuni | |
3.5.1.32 | 3.377 | - |
N-acetylglycine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 5.45 | - |
N-acetyl-L-alanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.32 | Ba2+ | 10% activation at 10 mM | Campylobacter jejuni | |
3.5.1.32 | K+ | 20% activation at 10 mM | Campylobacter jejuni | |
3.5.1.32 | Mg2+ | 20% activation at 10 mM | Campylobacter jejuni | |
3.5.1.32 | Zn2+ | 1.98 molecules of zinc per enzyme subunit molecule, metalloenzyme | Campylobacter jejuni |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.32 | 42400 | - |
4 * 42400, recombinant His6-tagged enzyme, SDS-PAGE | Campylobacter jejuni |
3.5.1.32 | 193000 | - |
recombinant His6-tagged enzyme, gel filtration | Campylobacter jejuni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.32 | hippurate + H2O | Campylobacter jejuni | - |
benzoate + glycine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.32 | Campylobacter jejuni | - |
strain ATCC 43431, gene hipO | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.32 | recombinant His6-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography | Campylobacter jejuni |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.1.32 | hippurate + H2O = benzoate + glycine | residues Asp76, Aps104, Glu134, Glu135, His161 and His356 are involved in zinc-binding and enzyme catalysis | Campylobacter jejuni |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.32 | 120 | - |
purified recombinant wild-type enzyme | Campylobacter jejuni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.32 | 4-aminobenzoyl-glutamate + H2O | - |
Campylobacter jejuni | 4-aminobenzoate + glutamate | - |
? | |
3.5.1.32 | hippurate + H2O | - |
Campylobacter jejuni | benzoate + glycine | - |
? | |
3.5.1.32 | hippurate + H2O | i.e. N-benzoylglycine | Campylobacter jejuni | benzoate + glycine | - |
? | |
3.5.1.32 | additional information | substrate specificity, the enzyme also shows metallocarboxypeptidase activity with carboxypeptidase substrates in vitro, displaying its highest activity against N-benzoyl-linked small aliphatic acids, overview, the enzyme does not hydrolyze typical substrates for aminopeptidases, lysine-p-nitroanilide, leucine-p-nitroanilide, or endopeptidases, N-benzoylarginine-p-nitroanilide, succinylphenylalanine-4-nitroanilide | Campylobacter jejuni | ? | - |
? | |
3.5.1.32 | N-acetylalanine + H2O | - |
Campylobacter jejuni | acetate + alanine | - |
? | |
3.5.1.32 | N-acetylglycine + H2O | - |
Campylobacter jejuni | acetate + glycine | - |
? | |
3.5.1.32 | N-benzoylalanine + H2O | - |
Campylobacter jejuni | benzoate + alanine | - |
? | |
3.5.1.32 | N-benzoylglutamate + H2O | - |
Campylobacter jejuni | benzoate + glutamate | - |
? | |
3.5.1.32 | N-benzoylmethionine + H2O | - |
Campylobacter jejuni | benzoate + methionine | - |
? | |
3.5.1.32 | N-benzoylphenylalanine + H2O | - |
Campylobacter jejuni | benzoate + phenylalanine | - |
? | |
3.5.1.32 | N-benzoylvaline + H2O | - |
Campylobacter jejuni | benzoate + valine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.32 | More | the enzyme structure shows highly ordered alpha-helix and beta-sheet sequences | Campylobacter jejuni |
3.5.1.32 | tetramer | 4 * 42400, recombinant His6-tagged enzyme, SDS-PAGE | Campylobacter jejuni |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.32 | hippurate hydrolase | - |
Campylobacter jejuni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.32 | 50 | - |
- |
Campylobacter jejuni |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.32 | 0.052 | 2.1 | N-acetylglycine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 0.17 | - |
N-benzoylglutamate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 0.5 | - |
N-benzoylvaline | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 1.4 | - |
hippurate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 2 | 8 | N-acetyl-L-alanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 2.32 | - |
hippurate | pH 7.5, 37°C, recombinant mutant D76N | Campylobacter jejuni | |
3.5.1.32 | 2.6 | - |
N-benzoylmethionine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 5.95 | - |
N-benzoylphenylalanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 10.18 | - |
N-acetylglycine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 13.33 | - |
N-benzoylvaline | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 14.75 | - |
4-aminobenzoyl-glutamate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 22.12 | - |
N-acetyl-L-alanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 29.38 | - |
N-benzoylalanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 48.72 | - |
N-benzoylglutamate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 57.13 | - |
N-benzoylmethionine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 81 | - |
N-benzoylalanine | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni | |
3.5.1.32 | 98.73 | - |
hippurate | pH 7.5, 37°C, purified recombinant wild-type enzyme | Campylobacter jejuni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.32 | 7.5 | - |
- |
Campylobacter jejuni |