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Literature summary extracted from

  • Moskovitz, J.; Flescher, E.; Berlett, B.S.; Azare, J.; Poston, J.M.; Stadtman, E.R.
    Overexpression of peptide-methionine sulfoxide reductase in Saccharomyces cerevisiae and human T cells provides them with high resistance to oxidative stress (1998), Proc. Natl. Acad. Sci. USA, 95, 14071-14075.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 gene msrA, subcloning in Escherichia coli, stable functional overexpression of MsrA in Saccharomyces cerevisiae and human T cells Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 additional information overexpression of peptide-methionine sulfoxide reductase in Saccharomyces cerevisiae, resulting in a strain with 25fold increased activity, and Molt-4 human lymphocyte cells provides them with high resistance to oxidative stress by H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride, the recombinant yeast strain grows better and and shows a higher survival rate compared to the wild-type parent strain Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 additional information Saccharomyces cerevisiae MsrA protects the cell against damage caused by oxidative stress through treatment with H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride ?
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin Saccharomyces cerevisiae
-
 peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Saccharomyces cerevisiae
-
 gene msrA
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.11 additional information
-
 cell survival rates after treatement with H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride Saccharomyces cerevisiae
1.8.4.11 0.000015
-
 wild-type Saccharomyces cerevisiae strain, substrate L-methionine-(S)-S-oxide Saccharomyces cerevisiae
1.8.4.11 0.000068
-
 wild-type Saccharomyces cerevisiae strain, substrate dabsyl-L-methionine-(S)-S-oxide Saccharomyces cerevisiae
1.8.4.11 0.0003
-
 recombinant Saccharomyces cerevisiae strain overexpressing MsrA, substrate L-methionine-(S)-S-oxide Saccharomyces cerevisiae
1.8.4.11 0.0017
-
 recombinant Saccharomyces cerevisiae strain overexpressing MsrA, substrate dabsyl-L-methionine-(S)-S-oxide Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 dabsyl-L-methionine-(S)-S-oxide + thioredoxin
-
 Saccharomyces cerevisiae dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 L-methionine-(S)-S-oxide + thioredoxin
-
 Saccharomyces cerevisiae L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 additional information MsrA protects the cell against damage caused by oxidative stress through treatment with H2O2, paraquat, or 2,2'-azobis-(2-amidinopropane) dihydrochloride Saccharomyces cerevisiae ?
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin
-
 Saccharomyces cerevisiae peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 MsrA
-
 Saccharomyces cerevisiae
1.8.4.11 peptide-methionine sulfoxide reductase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.11 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.11 7.5
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 thioredoxin
-
 Saccharomyces cerevisiae