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Literature summary extracted from

  • Ohnishi, M.; Mimura, T.; Tsujimura, T.; Mitsuhashi, N.; Washitani-Nemoto, S.; Maeshima, M.; Martinoia, E.
    Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status (2006), Planta, 225, 711-718.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 vacuolar membrane
-
 Catharanthus roseus 5774
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Mg2+ required Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Catharanthus roseus
-
 2 phosphate
-
 ?
3.6.1.1 additional information Catharanthus roseus the enzyme is a V-type vacuolar H+-pump and responsible for phosphate uptake across the vacuolar membrane, mechanism of activation of phosphate uptake into the vacuole under low phosphate status, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 partially by vacuole membrane-enriched fraction preparation Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.1 cell suspension culture
-
 Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 additional information
-
 activity of H+-PPase measured as K+/Na+-dependent diphosphate hydrolysis activity, phosphate uptake of vacuoles isolated from high phosphate and low phosphate cells, activity profiles, cellular phosphate content, overview Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Catharanthus roseus 2 phosphate
-
 ?
3.6.1.1 additional information the enzyme is a V-type vacuolar H+-pump and responsible for phosphate uptake across the vacuolar membrane, mechanism of activation of phosphate uptake into the vacuole under low phosphate status, overview Catharanthus roseus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 H+-PPase
-
 Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 7
-
 assay at Catharanthus roseus