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Literature summary extracted from

  • Kuzma, J.; Fall, R.
    Leaf isoprene emission rate is dependent on leaf development and the level of isoprene synthase (1993), Plant Physiol., 101, 435-440.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.27 additional information the enzyme is positively regulated by light Mucuna sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 Ca2+ stimulates at up to 2 mM, inhibits at 2-30 mM Mucuna sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Ca2+ stimulates at up to 2 mM, inhibits at 2-30 mM Mucuna sp.
4.2.3.27 Mg2+ dependent on, best at 15 mM, 20fold higher activity compared to Mn2+ Mucuna sp.
4.2.3.27 Mn2+ can partially substitute for Mg2+, 20fold lower activity compared to Mg2+ Mucuna sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Mucuna sp.
-
 isoprene + diphosphate isoprene is emmitted to the atmosphere ir

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Mucuna sp.
-
 velvet bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.27 native enzyme partially by ammonium sulfate fractionation and anion exchange chromatography Mucuna sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 leaf activity during leaf development, highest activity an isoprene emission in about 14-days-old leaves, overview Mucuna sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 additional information
-
 activity and isoprene emission during leaf development, overview Mucuna sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Mucuna sp. isoprene + diphosphate isoprene is emmitted to the atmosphere ir
4.2.3.27 dimethylallyl diphosphate
-
 Mucuna sp. isoprene + diphosphate i.e. 2-methyl-1,3-butadiene ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 30
-
 assay at Mucuna sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 7.8 8.5
-
 Mucuna sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.27 5.4 11.2
-
 Mucuna sp.