EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.116 | additional information | localization of seven biosynthetic enzymes to the sieve elements, unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy | Papaver somniferum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.102 | heterologous expression | Papaver somniferum |
2.1.1.116 | into pRSETB and introduced into Escherichia coli strain ER2566 pLys S | Papaver somniferum |
2.1.1.128 | expressed as His-tag fusion protein in Escherichia coli ER2566pLys S | Papaver somniferum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.247 | cytosol | - |
Papaver somniferum | 5829 | - |
1.14.14.102 | endoplasmic reticulum membrane | membrane-bound, in sieve element ER | Papaver somniferum | 5789 | - |
1.14.14.102 | additional information | subcellular localization study | Papaver somniferum | - |
- |
2.1.1.116 | cytoplasm | - |
Papaver somniferum | 5737 | - |
2.1.1.128 | cytoplasm | immunogold labelling studies show the strict association with electron dense regions of the peripheral cytoplasm of sieve elements | Papaver somniferum | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.102 | Fe2+ | P450-dependent hydroxylase | Papaver somniferum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.116 | 39400 | - |
immunoblot analysis | Papaver somniferum |
2.1.1.128 | 38500 | - |
x * 38500, immunoblotting | Papaver somniferum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.102 | (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 | Papaver somniferum | the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview | (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
2.1.1.128 | S-adenosyl-L-methionine + (S)-norcoclaurine | Papaver somniferum | putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis | S-adenosyl-L-homocysteine + (S)-coclaurine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.247 | Papaver somniferum | - |
opium poppy | - |
1.14.14.102 | Papaver somniferum | - |
cv. Louisiana | - |
1.21.3.3 | Papaver somniferum | - |
- |
- |
2.1.1.116 | Papaver somniferum | - |
cultivars Louisiana and Marianne | - |
2.1.1.128 | Papaver somniferum | - |
cv. Louisiana and Marianne | - |
2.1.1.140 | Papaver somniferum | - |
L. cv. Marianne and cv. Louisianne | - |
2.3.1.150 | Papaver somniferum | Q94FT4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.247 | Ni2+-charged HiTrap column chromatography | Papaver somniferum |
1.14.14.102 | recombinant enzyme | Papaver somniferum |
2.1.1.116 | by Ni2+ affinity chromatography | Papaver somniferum |
2.1.1.128 | recombinant protein using His-tag | Papaver somniferum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.247 | hypocotyl | - |
Papaver somniferum | - |
1.1.1.247 | root | - |
Papaver somniferum | - |
1.14.14.102 | phloem | localization in the sieve elements adjacent or proximal to laticifers in the root and hypocotyl of opium poppy seedlings | Papaver somniferum | - |
1.14.14.102 | root | phloem | Papaver somniferum | - |
1.14.14.102 | seedling | root phloem, the abundance of the enzyme increases rapidly between 1 and 3 days after seed germination | Papaver somniferum | - |
1.21.3.3 | hypocotyl | of seedling, localization in the sieve elelments | Papaver somniferum | - |
1.21.3.3 | root | of seedling, localization in the sieve elements | Papaver somniferum | - |
1.21.3.3 | seedling | sieve elements of root and hypocotyl, abundance of enzyme increases rapidly between 1 and 3 days of germination | Papaver somniferum | - |
2.1.1.116 | flower bud | higher levels of 4'OMT mRNA in cultivar Marianne, compared with cultivar Louisiana | Papaver somniferum | - |
2.1.1.116 | hypocotyl | - |
Papaver somniferum | - |
2.1.1.116 | leaf | lowest gene transcripts | Papaver somniferum | - |
2.1.1.116 | phloem | restricted to sieve elements adjacent or proximal to laticifers | Papaver somniferum | - |
2.1.1.116 | root | highest gene transcripts | Papaver somniferum | - |
2.1.1.116 | seedling | abundance of the enzyme increases rapidly between 1 and 3 days after seed germination | Papaver somniferum | - |
2.1.1.116 | stem | highest gene transcripts and levels of protein | Papaver somniferum | - |
2.1.1.128 | flower | low enzyme level | Papaver somniferum | - |
2.1.1.128 | leaf | low enzyme level | Papaver somniferum | - |
2.1.1.128 | additional information | gene transcripts detected in all organs with highest levels in root and stem and lowest in leaf | Papaver somniferum | - |
2.1.1.128 | phloem | sieve elements of the phloem adjacent or proximal to laticifers | Papaver somniferum | - |
2.1.1.128 | root | - |
Papaver somniferum | - |
2.1.1.128 | seed | constant enzyme level over 16 days of germination | Papaver somniferum | - |
2.1.1.128 | stem | - |
Papaver somniferum | - |
2.1.1.140 | phloem | sieve elements of the phloem adjacent or proximal to laticifers. Sieve elements in the root and hypocotyl of opium poppy seedlings | Papaver somniferum | - |
2.3.1.150 | phloem | enzyme protein is restricted to sieve elements of the phloem adjacent or proximal to laticifers. Corresponding gene transcripts are found in the companion cell paired with each sieve element | Papaver somniferum | - |
2.3.1.150 | seed | abundance of enzyme increases rapidly between 1 and 3 days of seed germination | Papaver somniferum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.247 | Codeinone + NADPH | - |
Papaver somniferum | Codeine + NADP+ | - |
? | |
1.1.1.247 | Morphinone + NADPH | - |
Papaver somniferum | Morphine + NADP+ | - |
? | |
1.14.14.102 | (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Papaver somniferum | (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.102 | (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 | the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview | Papaver somniferum | (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
2.1.1.128 | S-adenosyl-L-methionine + (S)-norcoclaurine | putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis | Papaver somniferum | S-adenosyl-L-homocysteine + (S)-coclaurine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.128 | ? | x * 38500, immunoblotting | Papaver somniferum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.247 | codeinone reductase | - |
Papaver somniferum |
1.1.1.247 | COR | - |
Papaver somniferum |
1.14.14.102 | (S)-N-methylcoclaurine 3'-hydroxylase | - |
Papaver somniferum |
1.14.14.102 | CYP80B1 | - |
Papaver somniferum |
2.1.1.116 | (S)-3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase | - |
Papaver somniferum |
2.1.1.116 | 4'OMT | - |
Papaver somniferum |
2.1.1.128 | (S)-norcoclaurine 6-O-methyltransferase | - |
Papaver somniferum |
2.1.1.128 | 6OMT | - |
Papaver somniferum |
2.1.1.140 | (S)-Coclaurine N-methyltransferase | - |
Papaver somniferum |
2.1.1.140 | CNMT | - |
Papaver somniferum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.247 | NADPH | - |
Papaver somniferum | |
1.14.14.102 | cytochrome P450 | P450-dependent hydroxylase | Papaver somniferum |