EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.19.62 | additional information | involved in vindoline biosynthesis | Catharanthus roseus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.103 | T16H expression analysis in leaves, overview, recombinant expression in Escherichia coli | Catharanthus roseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.103 | additional information | Catharanthus roseus | the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview | ? | - |
? | |
1.14.14.103 | tabersonine + NADPH + H+ + O2 | Catharanthus roseus | - |
16-hydroxytabersonine + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.103 | Catharanthus roseus | - |
- |
- |
1.14.19.62 | Catharanthus roseus | - |
- |
- |
3.2.1.105 | Catharanthus roseus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.103 | epidermal cell | predominantly | Catharanthus roseus | - |
1.14.14.103 | leaf | quantitative expression analysis in leaves | Catharanthus roseus | - |
1.14.14.103 | additional information | localization study | Catharanthus roseus | - |
1.14.19.62 | epidermal cell | leaf epidermal cells are biosynthetically competent to produce secologanin precursors that are converted via many enzymatic transformations to make 16-methoxytabersonine, which is then transported to cells within Catharanthus leaves to complete the last three or four enzymatic transformations to make vindoline | Catharanthus roseus | - |
1.14.19.62 | leaf | - |
Catharanthus roseus | - |
3.2.1.105 | leaf epidermis | abaxial and adaxial | Catharanthus roseus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.103 | additional information | the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview | Catharanthus roseus | ? | - |
? | |
1.14.14.103 | tabersonine + NADPH + H+ + O2 | - |
Catharanthus roseus | 16-hydroxytabersonine + NADP+ + H2O | - |
? | |
3.2.1.105 | strictosidine + H2O | The structural diversity of monoterpenoid indole alkaloids comes first from the deglucosylation of strictosidine by strictosidine beta-glucosidase. The unstable and versatile aglycone is biochemically converted by mostly uncharacterized enzyme reactions to the Corynanthe, Iboga and Aspidosperma classes of alkaloids such as ajmalicine, catharanthine and tabersonine, respectively | Catharanthus roseus | D-glucose + strictosidine aglycone | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.103 | T16H | - |
Catharanthus roseus |
3.2.1.105 | SGD | - |
Catharanthus roseus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.103 | 37 | - |
assay at | Catharanthus roseus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.103 | cytochrome P450 | - |
Catharanthus roseus |