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Literature summary extracted from

  • Gallo, C.M.; Smith, D.L.; Smith, J.S.
    Nicotinamide clearance by Pnc1 directly regulates Sir2-mediated silencing and longevity (2004), Mol. Cell. Biol., 24, 1301-1312.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.19 additional information stress suppresses the inhibitory effect of nicotinamide through the induction of PNC1 expression Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.19 gene PNC1, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.19 C167A site-directed mutagenesis, inactive mutant, no rescue the inhibition of NAD+-dependent histone deacetylase, i.e. HDAC, activity of Sir2 by nicotinamide Saccharomyces cerevisiae
3.5.1.19 additional information PNC1 overexpression suppresses the inhibitory effect of exogenously added nicotinamide on silencing, life span, and Hst1-mediated transcriptional repression Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Saccharomyces cerevisiae nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview nicotinate + NH3
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Saccharomyces cerevisiae
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 gene PNC1
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview Saccharomyces cerevisiae nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O Pnc1 stimulates Sir2, an NAD+-dependent histone deacetylase, and prevents the accumulation of nicotinamide Saccharomyces cerevisiae nicotinate + NH3
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 Pnc1
-
 Saccharomyces cerevisiae