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Literature summary extracted from

  • Watanabe, S.; Yasutake, Y.; Tanaka, I.; Takada, Y.
    Elucidation of stability determinants of cold-adapted monomeric isocitrate dehydrogenase from a psychrophilic bacterium, Colwellia maris, by construction of chimeric enzymes (2005), Microbiology, 151, 1083-1094.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 expressed in Escherichia coli Azotobacter vinelandii
1.1.1.41 expressed in Escherichia coli Colwellia maris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 5.8
-
 NADP+
-
 Azotobacter vinelandii
1.1.1.41 6.9
-
 NADP+
-
 Colwellia maris
1.1.1.41 7.9
-
 D-isocitrate
-
 Azotobacter vinelandii
1.1.1.41 62.2
-
 D-isocitrate
-
 Colwellia maris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mg2+
-
 Azotobacter vinelandii
1.1.1.41 Mg2+
-
 Colwellia maris
1.1.1.41 Mn2+
-
 Azotobacter vinelandii
1.1.1.41 Mn2+
-
 Colwellia maris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 80000
-
 1 * 80000, SDS-PAGE Azotobacter vinelandii
1.1.1.41 80000
-
 1 * 80000, SDS-PAGE Colwellia maris

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Azotobacter vinelandii
-
-
-
1.1.1.41 Colwellia maris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 Ni2+-affinity chromatography Azotobacter vinelandii
1.1.1.41 Ni2+-affinity chromatography Colwellia maris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.41 50.5
-
 with 0.12 mM NADP+ and 2 mM D-isocitrate Colwellia maris
1.1.1.41 75.5
-
 with 0.12 mM NADP+ and 2 mM D-isocitrate Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 D-isocitrate + NAD+
-
 Azotobacter vinelandii 2-oxoglutarate + CO2 + NADH
-
 r
1.1.1.41 D-isocitrate + NAD+
-
 Colwellia maris 2-oxoglutarate + CO2 + NADH
-
 r
1.1.1.41 isocitrate + NADP+
-
 Azotobacter vinelandii 2-oxoglutarate + CO2 + NADPH + H+
-
 r
1.1.1.41 isocitrate + NADP+
-
 Colwellia maris 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.41 monomer 1 * 80000, SDS-PAGE Azotobacter vinelandii
1.1.1.41 monomer 1 * 80000, SDS-PAGE Colwellia maris

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH
-
 Azotobacter vinelandii
1.1.1.41 IDH
-
 Colwellia maris
1.1.1.41 isocitrate dehydrogenase
-
 Azotobacter vinelandii
1.1.1.41 isocitrate dehydrogenase
-
 Colwellia maris
1.1.1.41 NAD(P)+-dependent isocitrate dehydrogenase
-
 Azotobacter vinelandii
1.1.1.41 NAD(P)+-dependent isocitrate dehydrogenase
-
 Colwellia maris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.41 25
-
-
 Colwellia maris
1.1.1.41 47.5
-
-
 Azotobacter vinelandii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.41 57.5
-
 D-isocitrate
-
 Colwellia maris
1.1.1.41 61.3
-
 NADP+
-
 Colwellia maris
1.1.1.41 73
-
 D-isocitrate
-
 Azotobacter vinelandii
1.1.1.41 92.6
-
 NADP+
-
 Azotobacter vinelandii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NADP+
-
 Azotobacter vinelandii
1.1.1.41 NADP+
-
 Colwellia maris