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Literature summary extracted from
- Heterologous expression of dehydroascorbate reductase from rice and its application to determination of dehydroascorbate concentrations (2006), J. Nutr. Sci. Vitaminol., 52, 89-95.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | expressed in Escherichia coli | Oryza sativa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.5.1 | Cyanate | 500 mM, 15% inhibition | Oryza sativa |  |
| 1.8.5.1 | iodoacetic acid | 41% and 1% residual activity after treatment with 0.1 mM and 1 mM, respectively | Oryza sativa | |
| 1.8.5.1 | additional information | heat treatment at 80°C for 10 min inhibits the enzyme leading to 1% residual activity | Oryza sativa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 0.35 | - |
dehydroascorbate | rice recombinant DHAR | Oryza sativa | |
| 1.8.5.1 | 1 | - |
glutathione | rice recombinant DHAR | Oryza sativa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.5.1 | cytosol | - |
Oryza sativa | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 26000 | - |
theoretical molecular weight calculated from sequence of cDNA | Oryza sativa |
| 1.8.5.1 | 27000 | - |
Ni2+-chelating NTA affinity chromatography, doublet polypeptide | Oryza sativa |
| 1.8.5.1 | 27000 | - |
SDS-PAGE, doublet polypeptide | Oryza sativa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.1 | Oryza sativa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | Ni2+-chealting NiNTA Superflow column chromatography | Oryza sativa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 49.1 | - |
rice cytosolic DHAR | Oryza sativa |
| 1.8.5.1 | 350 | - |
rice recombinant DHAR | Oryza sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | - |
Oryza sativa | GSSG + ascorbate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | dehydroascorbate reductase | - |
Oryza sativa |
| 1.8.5.1 | DHAR | - |
Oryza sativa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 80 | - |
heat treatment at 80°C for 10 min inhibits the enzyme leading to 1% residual activity | Oryza sativa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 8.2 | - |
- |
Oryza sativa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 6 | 8 | the activity of the enzyme at pH 6 was only 20% of that at pH 8.0 | Oryza sativa |
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Release 2023.1 (January 2023)
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