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Literature summary extracted from

  • Sidyelyeva, G.; Baker, N.E.; Fricker, L.D.
    Characterization of the molecular basis of the Drosophila mutations in carboxypeptidase D. Effect on enzyme activity and expression (2006), J. Biol. Chem., 281, 13844-13852.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.17.22 additional information Drosophila CPD is encoded by the silver gene svr, which is differentially spliced to produce long transmembrane protein forms with three metallocarboxypeptidase-like domains and short soluble forms with a single metallocarboxypeptidase domain. Flies that retain the short form are viable, whereas flies that are missing all forms of CPD do not survive past the early larval stages Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.22 baculovirus expression of constructs containing either the 1A or the 1B form and one of three different sequences of domain 2 of CPD (wild type, mutants svr1, or svrpoi) in Sf9 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.4.16.6 additional information enzyme is encoded by silver gene svr. Mutant lines svrPG33 do not survive past the early larval stage, having a P-element insertion upstream of the initiation ATG. Mutant lines svr1 and svrpoi are viable, with silvery body color and poited wings. svr1 gene has a three-nucleotide deletion in exon 6, svrpoi has a 1072-bp duplication of the gene that introduces a stop codon into the open reading frame. Both mutations eliminate enzyme activity of the second carboxypeptidase-like domain, which reduces levels of the long forms of enzyme but does not affect the levels of the short forms Drosophila melanogaster
3.4.17.22 additional information svrPG33 mutants, do not survive past the early larval stage. These mutants have a P-element insertion within exon 1B upstream of the initiation ATG, which greatly reduces mRNA levels of all forms of CPD. Both svr1 and svrpoi mutants are viable, both deletions eliminate enzyme activity of the second CP-like domain and appear to cause the misfolding of the protein. This greatly reduces the levels of the long forms of CPD protein but do not affect the levels of the short forms Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.17.22 membrane
-
 Drosophila melanogaster 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.22 150000
-
 antisera raised against the N-terminal region of the 1A form and the C-terminal region of the tail-2 form of CPD Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.6 Drosophila melanogaster
-
 enzyme encoded by silver gene svr
-
3.4.17.22 Drosophila melanogaster P42787
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.22 on affinity resin Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.4.17.22 Carboxypeptidase D
-
 Drosophila melanogaster
3.4.17.22 CPD
-
 Drosophila melanogaster