EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.25 | His-tagged R265H mutant, expressed in E. coli BL21 | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.25 | R265H | no AdoMet synthetase activity, normal tripolyphosphatase activity | Rattus norvegicus |
3.6.1.25 | R265S | no AdoMet synthetase activity, reduced tripolyphosphatase activity | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.25 | additional information | - |
additional information | no change in activity after nitrosylation of the enzyme with 250 microM nitrosylated glutathione | Rattus norvegicus | |
3.6.1.25 | 0.02 | - |
tripolyphosphate | wildtype, 2 mM substrate | Rattus norvegicus | |
3.6.1.25 | 0.078 | - |
tripolyphosphate | R265H mutant, 2 mM substrate in the presence of 5 mM ATP | Rattus norvegicus | |
3.6.1.25 | 0.083 | - |
tripolyphosphate | R265H mutant, 2 mM substrate | Rattus norvegicus | |
3.6.1.25 | 0.122 | - |
tripolyphosphate | R265S mutant, 2 mM substrate | Rattus norvegicus | |
3.6.1.25 | 0.143 | - |
tripolyphosphate | R265H mutant, 2 mM substrate in the presence of 5 mM diphosphate | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.25 | 41600 | - |
R265H mutant, gel filtration, column equilibrated with 2 mM tripolyphosphate | Rattus norvegicus |
3.6.1.25 | 90000 | - |
heterodimer of R265H mutant and wildtype enzyme, size exclusion chromatography | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.25 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.25 | His-tagged R265H mutant purified with Ni2+ Sepharose column chromatography and wildtype enzyme purified from liver | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.25 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.25 | additional information | - |
tripolyphosphate activity of R265H mutant is specific for tripolyphosphate and depends on magnesium ions, potassium is not required | Rattus norvegicus |
3.6.1.25 | 0.001 | - |
R265S mutant, 2 mM methionine and 2 mM ATP as substrate | Rattus norvegicus |
3.6.1.25 | 0.0012 | - |
tripolyphosphatase activity of R265H mutant, 2 mM ATP as substrate | Rattus norvegicus |
3.6.1.25 | 0.0019 | - |
tripolyphosphatase activity of R265H mutant, 2 mM pyrophosphate as substrate | Rattus norvegicus |
3.6.1.25 | 0.002 | - |
R265H mutant, 2 mM methionine and 2 mM ATP as substrate | Rattus norvegicus |
3.6.1.25 | 0.004 | - |
tripolyphosphatase activity of R265H mutant, 2 mM metatripolyphosphate as substrate | Rattus norvegicus |
3.6.1.25 | 0.029 | - |
tripolyphosphatase activity of R265S mutant, 2 mM tripolyphosphate as substrate | Rattus norvegicus |
3.6.1.25 | 0.067 | - |
tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate in the presence of 5 mM pyrophosphate | Rattus norvegicus |
3.6.1.25 | 0.128 | - |
tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate | Rattus norvegicus |
3.6.1.25 | 0.129 | - |
tripolyphosphatase activity of R265H mutant, 2 mM tripolyphosphate as substrate in the presence of 5 mM ATP | Rattus norvegicus |
3.6.1.25 | 0.156 | - |
tripolyphosphatase activity of wildtype, 2 mM tripolyphosphate as substrate | Rattus norvegicus |
3.6.1.25 | 0.588 | - |
wildtype, 2 mM methionine and 2 mM ATP as substrate | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.25 | triphosphate + H2O | - |
Rattus norvegicus | diphosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.25 | heterodimer | containing one R265H mutant subunit and one wildtype subunit, impaired AdoMet synthetase activity | Rattus norvegicus |
3.6.1.25 | monomer | R265H mutant | Rattus norvegicus |