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Literature summary extracted from

  • Edgar, J.R.; Bell, R.M.
    Biosynthesis in Escherichia coli of sn-glycerol-3-phosphate, a precursor of phospholipid. Further kinetic characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycerol-3-phosphate dehydrogenase (1980), J. Biol. Chem., 255, 3492-3497.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.94 additional information existence of a naturally occurring feedback inhibition-resistant mutant Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.94 2',5'-ADP
-
 Escherichia coli
1.1.1.94 3,4-dihydroxybutyl 1-phosphonate competitive versus dihydroxyacetone phosphate, binding to a regulatory site Escherichia coli
1.1.1.94 ADP
-
 Escherichia coli
1.1.1.94 ADP-ribose
-
 Escherichia coli
1.1.1.94 AMP
-
 Escherichia coli
1.1.1.94 ATP
-
 Escherichia coli
1.1.1.94 ethylene glycol phosphate binding to the active site Escherichia coli
1.1.1.94 NAD+
-
 Escherichia coli
1.1.1.94 NADP+
-
 Escherichia coli
1.1.1.94 NMN
-
 Escherichia coli
1.1.1.94 sn-glycerol-3-phosphate the wild-type enzyme is feedback regulated, competitive versus dihydroxyacetone phosphate, binding to a regulatory site Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.94 0.0037
-
 NADPH pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 0.004
-
 NADH pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 0.0041
-
 NADPH pH 7.4, 23°C, wild-type mutant enzyme Escherichia coli
1.1.1.94 0.0045
-
 NADH pH 7.4, 23°C, wild-type mutant enzyme Escherichia coli
1.1.1.94 0.005
-
 reduced nicotinamide hypoxanthine dinucleotide pH 7.4, 23°C, wild-type mutant enzyme and feedback-resistant mutant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.94 glycerone phosphate + NAD(P)H Escherichia coli
-
 sn-glycerol-3-phosphate + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.94 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.94 glycerone phosphate + NAD(P)H
-
 Escherichia coli sn-glycerol-3-phosphate + NAD(P)+
-
 r
1.1.1.94 glycerone phosphate + NAD(P)H i.e. dihydroxyacetone phosphate Escherichia coli sn-glycerol-3-phosphate + NAD(P)+ product analysis r
1.1.1.94 glycerone phosphate + NADH + H+
-
 Escherichia coli sn-glycerol 3-phosphate + NAD+
-
 ?
1.1.1.94 glycerone phosphate + reduced nicotinamide hypoxanthine dinucleotide
-
 Escherichia coli sn-glycerol-3-phosphate + oxidized nicotinamide hypoxanthine dinucleotide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.94 sn-glycerol-3-phosphate dehydrogenase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.94 23
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.94 7.4
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.94 NAD+
-
 Escherichia coli
1.1.1.94 NADH
-
 Escherichia coli
1.1.1.94 NADP+ B-type specificity of wild-type and feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 NADPH B-type specificity of wild-type and feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 nicotinamide hypoxanthine dinucleotide
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.94 additional information
-
 additional information product inhibition kinetics Escherichia coli
1.1.1.94 0.1
-
 ADP-ribose pH 7.4, 23°C, wild-type enzyme Escherichia coli
1.1.1.94 0.19
-
 NADP+ pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 0.2
-
 NAD+ pH 7.4, 23°C, wild-type enzyme Escherichia coli
1.1.1.94 0.21
-
 ADP-ribose pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 0.22
-
 NAD+ pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 0.25
-
 NADP+ pH 7.4, 23°C, wild-type enzyme Escherichia coli
1.1.1.94 0.7
-
 ADP pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 0.8
-
 ADP pH 7.4, 23°C, wild-type enzyme Escherichia coli
1.1.1.94 1.4
-
 ethylene glycol phosphate pH 7.4, 23°C, wild-type and feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 1.4
-
 ATP pH 7.4, 23°C, wild-type enzyme and feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 1.8
-
 2',5'-ADP pH 7.4, 23°C, wild-type enzyme Escherichia coli
1.1.1.94 2
-
 2',5'-ADP pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 4.8
-
 AMP pH 7.4, 23°C, feedback-resistant mutant enzyme Escherichia coli
1.1.1.94 5
-
 AMP pH 7.4, 23°C, wild-type enzyme Escherichia coli
1.1.1.94 10
-
 NMN pH 7.4, 23°C, wild-type enzyme and feedback-resistant mutant enzyme Escherichia coli