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Literature summary extracted from

  • Mitsch, M.J.; Cowie, A.; Finan, T.M.
    Malic Enzyme Cofactor and Domain Requirements for Symbiotic N2-fixation by Sinorhizobium meliloti (2006), J. Bacteriol., 189, 160-168.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 gene tme, subcloning in Escherichia coli, expression in bacteroids deficient for the NAD+-dependent malic enzyme encoded by gene dme, EC 1.1.1.39 Sinorhizobium meliloti

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.39 additional information expression of the tme gene, EC 1.1.1.40, under the control of the dme promoter, cannot restore the N2 fixation activity which is lost in dme mutant cells in alfalfa root nodules, despite elevated levels of TME within bacteroids, no symbiotic nitrogen fixation occurs in dme mutant strains, overview Sinorhizobium meliloti
1.1.1.40 additional information the tme gene is placed under the control of the dme promoter, and despite elevated levels of TME within bacteroids, no symbiotic nitrogen fixation occurred in dme mutant strains, the dme mutant cells fail to fix N2 in alfalfa root nodules, overview, no TME in the tme mutant RmG994 Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.39 (S)-malate + NAD+ Sinorhizobium meliloti the enzyme plays a role in symbiotic N2 fixation, overview pyruvate + CO2 + NADH
-
 r
1.1.1.40 (S)-malate + NADP+ Sinorhizobium meliloti
-
 pyruvate + CO2 + NADPH
-
 r
1.1.1.40 additional information Sinorhizobium meliloti TME is not capable of N2 fixation probably due to a constantly high ratio of NADPH + H+ to NADP+ in nitrogen-fixing bacteroids, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Sinorhizobium meliloti
-
-
-
1.1.1.40 Sinorhizobium meliloti
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 bacteroid the level of the enzyme in wild-type bacteroids is not limiting for N2-fixation Sinorhizobium meliloti
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.40 0.008 0.89 enzyme activity in several recombinant strains, overview Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NAD+
-
 Sinorhizobium meliloti pyruvate + CO2 + NADH
-
 r
1.1.1.39 (S)-malate + NAD+ the enzyme plays a role in symbiotic N2 fixation, overview Sinorhizobium meliloti pyruvate + CO2 + NADH
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Sinorhizobium meliloti pyruvate + CO2 + NADPH
-
 r
1.1.1.40 additional information TME is not capable of N2 fixation probably due to a constantly high ratio of NADPH + H+ to NADP+ in nitrogen-fixing bacteroids, overview Sinorhizobium meliloti ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.39 diphosphate nucleotide dependent malic enzyme
-
 Sinorhizobium meliloti
1.1.1.39 DME
-
 Sinorhizobium meliloti
1.1.1.39 NAD+-dependent malic enzyme
-
 Sinorhizobium meliloti
1.1.1.40 NADP+-dependent malic enzyme
-
 Sinorhizobium meliloti
1.1.1.40 TME
-
 Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+
-
 Sinorhizobium meliloti
1.1.1.39 NADH
-
 Sinorhizobium meliloti
1.1.1.40 NADP+
-
 Sinorhizobium meliloti
1.1.1.40 NADPH
-
 Sinorhizobium meliloti