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Literature summary extracted from

  • Shapir, N.; Sadowsky, M.J.; Wackett, L.P.
    Purification and characterization of allophanate hydrolase (AtzF) from Pseudomonas sp. strain ADP (2005), J. Bacteriol., 187, 3731-3738.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.54 gene atzF, expression of the His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.54 K91A site-directed mutagenesis, inactive mutant Pseudomonas sp.
3.5.1.54 S165A site-directed mutagenesis, inactive mutant Pseudomonas sp.
3.5.1.54 S189A site-directed mutagenesis, inactive mutant Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.54 phenyl phosphorodiamidate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.54 1.5
-
 allophanate pH 9.5, recombinant enzyme Pseudomonas sp.
3.5.1.54 5.3
-
 malonamic acid pH 9.5, recombinant enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.54 66223
-
 4 * 66223, sequence calculation Pseudomonas sp.
3.5.1.54 260000
-
 gel filtration, recombinant enzyme Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.54 allophanate + H2O Pseudomonas sp.
-
 NH3 + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.54 Pseudomonas sp. Q936X2 gene atzF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.54 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.54 additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.54 allophanate + H2O
-
 Pseudomonas sp. NH3 + CO2
-
 ?
3.5.1.54 allophanate + H2O
-
 Pseudomonas sp. NH3 + CO2
-
 ir
3.5.1.54 biuret + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.54 malonamic acid + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.54 malonamic acid + hydroxylamine hydroxylamine trapping activity Pseudomonas sp. malonohydroxamate + ?
-
 ?
3.5.1.54 additional information substrate specificity, no activity with methyl allophanate, hydantoic acid, oxamic acid, hydroxyurea, methyl carbamate, N-methylurea, acetylurea, 1-acetyl-2-thiourea, and semicarbazide, no activity with rhodanine, rhodanine-3-acetic acid, 3-aminorhodanine, (4R)-(-)-2-thioxo-4-thiazolidinecarboxylic acid, (-)-2-oxo-4-thiazolinecarboxylic acid, and 2-amino-5-bromothiazol Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.54 tetramer 4 * 66223, sequence calculation Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.54 AtzF
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.54 0.1
-
 malonamic acid pH 9.5, recombinant enzyme Pseudomonas sp.
3.5.1.54 16.4
-
 allophanate pH 9.5, recombinant enzyme Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.54 9.5
-
 recombinant enzyme Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.54 8 11.3 recombinant enzyme Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.54 3.2
-
 phenyl phosphorodiamidate pH 9.5, recombinant enzyme Pseudomonas sp.