EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.5 | Bacillus subtilis gene ytkD cloned and expressed in Escherichia coli | Bacillus subtilis |
3.6.1.15 | gene ytkD, expression in Escherichia coli strain HMS174, complementation of the mutT mutator phenotype in Escherichia coli strain SB3 lacking a functional mutT gene | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.5 | 0.16 | - |
dGTP | pH 8.5, 37°C | Bacillus subtilis | |
3.6.1.5 | 0.43 | - |
8-oxo-dGTP | pH 8.5, 37°C | Bacillus subtilis | |
3.6.1.5 | 0.89 | - |
dATP | pH 8.5, 37°C | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.5 | Mg2+ | - |
Bacillus subtilis | |
3.6.1.5 | Mn2+ | 15% residual activity compared with Mg2+ | Bacillus subtilis | |
3.6.1.15 | Mg2+ | - |
Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.5 | Bacillus subtilis | - |
- |
- |
3.6.1.15 | Bacillus subtilis | - |
gene ytkD | - |
3.6.1.15 | Bacillus subtilis 168 | - |
gene ytkD | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.15 | recombinant enzyme from Escherichia coli strain HMS174 by streptomycin sulfate treatment and gel filtration | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.5 | 8-oxo-dGTP + 2 H2O | - |
Bacillus subtilis | 8-oxo-dGMP + 2 phosphate | - |
? | |
3.6.1.5 | adenosine (5')-tetraphospho-(5')-adenosine + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.6.1.5 | ADP-ribose + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.6.1.5 | ATP + 2 H2O | - |
Bacillus subtilis | AMP + 2 phosphate | - |
? | |
3.6.1.5 | CDP-choline + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.6.1.5 | CDP-choline + H2O | - |
Bacillus subtilis 168 | ? | - |
? | |
3.6.1.5 | CTP + 2 H2O | - |
Bacillus subtilis | CMP + 2 phosphate | - |
? | |
3.6.1.5 | dATP + H2O | - |
Bacillus subtilis | dAMP + phosphate + H+ | - |
? | |
3.6.1.5 | dCTP + H2O | - |
Bacillus subtilis | dCMP + phosphate + H+ | - |
? | |
3.6.1.5 | dGTP + H2O | - |
Bacillus subtilis | dGMP + phosphate + H+ | - |
? | |
3.6.1.5 | dTTP + H2O | - |
Bacillus subtilis | dTMP + phosphate + H+ | - |
? | |
3.6.1.5 | dUTP + H2O | - |
Bacillus subtilis | dUMP + phosphate + H+ | - |
? | |
3.6.1.5 | FAD + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.6.1.5 | FAD + H2O | - |
Bacillus subtilis 168 | ? | - |
? | |
3.6.1.5 | GDP-mannose + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.6.1.5 | GDP-mannose + H2O | - |
Bacillus subtilis 168 | ? | - |
? | |
3.6.1.5 | GTP + 2 H2O | - |
Bacillus subtilis | GMP + 2 phosphate | - |
? | |
3.6.1.5 | NADH + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.6.1.5 | UDP-glucose + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.6.1.5 | UTP + 2 H2O | - |
Bacillus subtilis | UMP + 2 phosphate | - |
? | |
3.6.1.5 | UTP + 2 H2O | - |
Bacillus subtilis 168 | UMP + 2 phosphate | - |
? | |
3.6.1.15 | dNTP + H2O | - |
Bacillus subtilis | dNDP + phosphate | - |
? | |
3.6.1.15 | dNTP + H2O | - |
Bacillus subtilis 168 | dNDP + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.15 | More | the enzyme is a member of the Nudix hydrolase superfamily | Bacillus subtilis |
3.6.1.15 | nucleoside triphosphatase | - |
Bacillus subtilis |
3.6.1.15 | YtkD | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | 37 | - |
assay at | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.5 | 0.74 | - |
dGTP | pH 8.5, 37°C | Bacillus subtilis | |
3.6.1.5 | 0.96 | - |
8-oxo-dGTP | pH 8.5, 37°C | Bacillus subtilis | |
3.6.1.5 | 1.5 | - |
dATP | pH 8.5, 37°C | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | 8.5 | - |
assay at | Bacillus subtilis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.5 | 1.6 | - |
dATP | pH 8.5, 37°C | Bacillus subtilis | |
3.6.1.5 | 2.4 | - |
8-oxo-dGTP | pH 8.5, 37°C | Bacillus subtilis | |
3.6.1.5 | 4.6 | - |
dGTP | pH 8.5, 37°C | Bacillus subtilis |