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Literature summary extracted from

  • Gao, H.; Leary, J.; Carroll, K.S.; Bertozzi, C.R.; Chen, H.
    Noncovalent Complexes of APS Reductase from M. tuberculosis: Delineating a Mechanistic Model Using ESI-FTICR MS (2007), J. Am. Soc. Mass Spectrom., 18, 167-178.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.10 Iron 4Fe-4S containing protein. Oxidation state of +2 for the 4Fe-4S cluster, with no disulfide bond in the holoenzyme Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 28350
-
calculated average mass, determined on the elemental composition of the apoprotein plus the components in the metal center, assuming that all cysteines are in reduced form and the metal cluster has an oxidation state of zero Mycobacterium tuberculosis
1.8.99.2 28360
-
calculated from amino acid sequence Mycobacterium tuberculosis
1.8.99.2 28700
-
1 * 28700, ESI-FTICR mass spectrometry Mycobacterium tuberculosis
1.8.99.2 28710
-
ESI-FTICR mass spectrometry Mycobacterium tuberculosis
1.8.99.2 28710
-
theoretical value based on the chemical formula Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.10 Mycobacterium tuberculosis
-
recombinantly expressed in Escherichia coli
-
1.8.99.2 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.10 adenosine 5'-phosphosulfate + thioredoxin noncovalent complexes of the holoprotein with several ligands, including adenosine 5'-phosphosulfate, thioredoxin, and AMP, are investigated. AMP binds with a higher affinity to the enzyme intermediate than to the free enzyme Mycobacterium tuberculosis AMP + sulfite + thioredoxin disulfide
-
?
1.8.99.2 adenylyl sulfate + reduced thioredoxin
-
Mycobacterium tuberculosis AMP + sulfite + oxidized thioredoxin
-
?

Subunits

EC Number Subunits Comment Organism
1.8.99.2 monomer 1 * 28700, ESI-FTICR mass spectrometry Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.8.4.10 APS reductase
-
Mycobacterium tuberculosis
1.8.99.2 adenosine 5'-phosphosulfate reductase
-
Mycobacterium tuberculosis
1.8.99.2 APR
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.2 thioredoxin
-
Mycobacterium tuberculosis