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Literature summary extracted from

  • Godoy, L.; Gonzalez-Duarte, R.; Albalat, R.
    S-Nitrosogluthathione reductase activity of amphioxus ADH3: insights into the nitric oxide metabolism (2006), Int. J. Biol. Sci., 2, 117-124.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.284 expressed in Escherichia coli BL21 (DE3) cells Branchiostoma floridae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.004
-
 S-hydroxymethylglutathione in 0.1 M sodium diphosphate at pH 8.0 Branchiostoma floridae
1.1.1.284 0.008
-
 NAD+ in 0.1 M sodium diphosphate at pH 7.5 Branchiostoma floridae
1.1.1.284 0.045
-
 NADH in 0.1 M sodium diphosphate at pH 7.5 Branchiostoma floridae
1.1.1.284 0.056
-
 S-nitrosoglutathione in 0.1 M sodium diphosphate at pH 8.0 Branchiostoma floridae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Branchiostoma floridae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284 Talon metal affinity resin chromatography Branchiostoma floridae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.284 gut
-
 Branchiostoma floridae
-
1.1.1.284 additional information dorsal region of the club-shaped gland Branchiostoma floridae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 S-hydroxymethylglutathione + NAD+
-
 Branchiostoma floridae S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 S-nitrosoglutathione + NADH
-
 Branchiostoma floridae GSH + NAD+ + NO
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 ADH3
-
 Branchiostoma floridae
1.1.1.284 alcohol dehydrogenase class III
-
 Branchiostoma floridae
1.1.1.284 FALDH
-
 Branchiostoma floridae
1.1.1.284 Glutathione-dependent formaldehyde dehydrogenase
-
 Branchiostoma floridae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 2.5
-
 S-hydroxymethylglutathione
-
 Branchiostoma floridae
1.1.1.284 16.7
-
 S-nitrosoglutathione
-
 Branchiostoma floridae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Branchiostoma floridae