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Literature summary extracted from

  • Kikuchi, H.; Kim, S.; Watanabe, K.; Watarai, M.
    Brucella abortus d-alanyl-d-alanine carboxypeptidase contributes to its intracellular replication and resistance against nitric oxide (2006), FEMS Microbiol. Lett., 259, 120-125.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.16.4 medicine Brucella spp. cause abortion in domestic animals and undulant fever in humans, DAP contributes to resistance against NO, which is required for the intracellular growth of the bacterium Brucella abortus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.17.14 additional information the enzyme mutant K23 of Brucella abortus fails to replicate in mouse macrophages and HeLa cells, and shows less virulence than the wild type in mice, Under nitric oxide (NO) stress, the growth of the DAP mutant in vitro decreases, and it also has less capability to reduce NO than the wild type, intracellular replication of the DAP mutant is partially restored by pretreatment of macrophages with the NO synthase inhibitor 1-phenyl-imidazole and the level of expression of the NO reductase gene norB in the DAP mutant is lower than that in the wild type Brucella abortus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.14 additional information Brucella abortus DAP contributes to resistance against NO required for the intracellular growth of the bacterium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.4 Brucella abortus
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 544 Brucella abortus biovar 1 strain and strain K23
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3.4.17.14 Brucella abortus
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 strain 544, ATCC 23448, a smooth virulent biovar 1 strain, gene dacF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.4 by RNA purification kit Brucella abortus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.14 additional information intracellular replication of Brucella abortus within murine macrophages and HeLa cells Brucella abortus
-

Storage Stability

EC Number Storage Stability Organism
3.4.16.4 -75°C Brucella abortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.14 additional information DAP contributes to resistance against NO required for the intracellular growth of the bacterium Brucella abortus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.16.4 D-alanyl-D-alanine carboxypeptidase
-
 Brucella abortus
3.4.16.4 DAP
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 Brucella abortus
3.4.17.14 D-alanyl-D-alanine carboxypeptidase
-
 Brucella abortus
3.4.17.14 DAP
-
 Brucella abortus