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Literature summary extracted from

  • Han, C.; Wang, L.; Yu, K.; Chen, L.; Hu, L.; Chen, K.; Jiang, H.; Shen, X.
    Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori (2006), FEBS J., 273, 4682-4692.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 gene aroE, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.25 2-([2-([2-([2-(2,3-dimethylanilino)-2-oxoethyl]sulfanyl)-1,3-benzothiazol-6-yl]amino)2-oxoethyl]sulfanyl)-N-(2-naphthyl)acetamide IC50: 0.0029 mM, competitive inhibition with respect to shikimate, noncompetitive to NADP+, potent antibacterial activity Helicobacter pylori
1.1.1.25 3-(2-naphthyloxy)-4-oxo-2-(trifluoromethyl)-4H-chromen-7-yl 3-chlorobenzoate IC50: 0.0039 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+ Helicobacter pylori
1.1.1.25 butyl 2-([3-(2-naphthyloxy)4-oxo-2-(trifluoromethyl)4H-chromen-7-yl]oxy)propanoate IC50: 0.0134 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+, potent antibacterial activity Helicobacter pylori
1.1.1.25 curcumin IC50: 0.0154 mM, noncompetitive inhibition with respect to shikimate and NADP+ Helicobacter pylori
1.1.1.25 maesaquinone diacetate IC50: 0.0035 mM, noncompetitive inhibition with respect to shikimate and NADP+ Helicobacter pylori
1.1.1.25 additional information antibacterial activity of inhibitors, overview Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.148
-
shikimate pH 8.0, 60°C, recombinant enzyme Helicobacter pylori
1.1.1.25 0.182
-
NADP+ pH 8.0, 60°C, recombinant enzyme Helicobacter pylori
1.1.1.25 2.9
-
NAD+ pH 8.0, 60°C, recombinant enzyme Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 3-dehydroshikimate + NAD(P)H + H+ Helicobacter pylori fourth enzyme involved in the shikimate pathway shikimate + NAD(P)+
-
r
1.1.1.25 3-dehydroshikimate + NAD(P)H + H+ Helicobacter pylori SS1 fourth enzyme involved in the shikimate pathway shikimate + NAD(P)+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Helicobacter pylori Q56S04 gene aroE; gene aroE
-
1.1.1.25 Helicobacter pylori SS1 Q56S04 gene aroE; gene aroE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.25 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Helicobacter pylori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.25 additional information
-
-
Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 3-dehydroshikimate + NAD(P)H + H+
-
Helicobacter pylori shikimate + NAD(P)+
-
r
1.1.1.25 3-dehydroshikimate + NAD(P)H + H+ fourth enzyme involved in the shikimate pathway Helicobacter pylori shikimate + NAD(P)+
-
r
1.1.1.25 3-dehydroshikimate + NAD(P)H + H+
-
Helicobacter pylori SS1 shikimate + NAD(P)+
-
r
1.1.1.25 3-dehydroshikimate + NAD(P)H + H+ fourth enzyme involved in the shikimate pathway Helicobacter pylori SS1 shikimate + NAD(P)+
-
r
1.1.1.25 quinate + NADP+
-
Helicobacter pylori 3-dehydroquinate + NADPH + H+
-
r
1.1.1.25 quinate + NADP+
-
Helicobacter pylori SS1 3-dehydroquinate + NADPH + H+
-
r
1.1.1.25 shikimate + NAD+
-
Helicobacter pylori 3-dehydroshikimate + NADH + H+
-
r
1.1.1.25 shikimate + NAD+
-
Helicobacter pylori SS1 3-dehydroshikimate + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.25 More analysis of secondary structure Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 SDH
-
Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 60
-
oxidation reaction, recombinant His-tagged enzyme Helicobacter pylori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.25 20 80
-
Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.25 5.2
-
NAD+ pH 8.0, 60°C, recombinant enzyme Helicobacter pylori
1.1.1.25 7.1
-
NADP+ pH 8.0, 60°C, recombinant enzyme Helicobacter pylori
1.1.1.25 7.7
-
shikimate pH 8.0, 60°C, recombinant enzyme Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 8 9 oxidation reaction, recombinant His-tagged enzyme Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.25 additional information
-
pH profile of the oxidation reaction Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NAD+
-
Helicobacter pylori
1.1.1.25 NADH
-
Helicobacter pylori
1.1.1.25 NADP+ preferred cofactor Helicobacter pylori
1.1.1.25 NADPH preferred cofactor Helicobacter pylori

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.25 0.0029
-
IC50: 0.0029 mM, competitive inhibition with respect to shikimate, noncompetitive to NADP+, potent antibacterial activity Helicobacter pylori 2-([2-([2-([2-(2,3-dimethylanilino)-2-oxoethyl]sulfanyl)-1,3-benzothiazol-6-yl]amino)2-oxoethyl]sulfanyl)-N-(2-naphthyl)acetamide
1.1.1.25 0.0035
-
IC50: 0.0035 mM, noncompetitive inhibition with respect to shikimate and NADP+ Helicobacter pylori maesaquinone diacetate
1.1.1.25 0.0039
-
IC50: 0.0039 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+ Helicobacter pylori 3-(2-naphthyloxy)-4-oxo-2-(trifluoromethyl)-4H-chromen-7-yl 3-chlorobenzoate
1.1.1.25 0.0134
-
IC50: 0.0134 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+, potent antibacterial activity Helicobacter pylori butyl 2-([3-(2-naphthyloxy)4-oxo-2-(trifluoromethyl)4H-chromen-7-yl]oxy)propanoate
1.1.1.25 0.0154
-
IC50: 0.0154 mM, noncompetitive inhibition with respect to shikimate and NADP+ Helicobacter pylori curcumin