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Literature summary extracted from

  • Mee, B.; Kelleher, D.; Frias, J.; Malone, R.; Tipton, K.F.; Henehan, G.T.; Windle, H.J.
    Characterization of cinnamyl alcohol dehydrogenase of Helicobacter pylori. An aldehyde dismutating enzyme (2005), FEBS J., 272, 1255-1264.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 gene CAD or HP1140 from strain 26995, cloning in Escherichia coli strain DH5alpha, and expression in strain BL21(DE3) as N-terminally His-tagged enzyme Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.195 benzyl alcohol competitive mechanism, substrate inhibition Helicobacter pylori
1.1.1.195 cinnamyl alcohol competitive mechanism, substrate inhibition Helicobacter pylori
1.1.1.195 coniferyl alcohol competitive mechanism, substrate inhibition Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.005
-
 cinnamyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 0.008
-
 coniferyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 0.03
-
 benzaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 0.04
-
 acetaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 0.06
-
 NADP+ pH 7.5, 37°C, recombinant enzyme, with benzyla alcohol Helicobacter pylori
1.1.1.195 0.1
-
 cinnamyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 0.11
-
 coniferyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 0.15
-
 NADPH pH 7.5, 37°C, recombinant enzyme, with acetaldehyde Helicobacter pylori
1.1.1.195 0.41
-
 benzyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 9
-
 butanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 13
-
 Propanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 31
-
 benzaldehyde pH 7.5, 37°C, recombinant enzyme, dismutase reaction with NADP+ Helicobacter pylori
1.1.1.195 46
-
 ethanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.195 cytosol predominantly Helicobacter pylori 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.195 cinnamyl alcohol + NADP+ Helicobacter pylori
-
 cinnamyl aldehyde + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Helicobacter pylori
-
 strains 1061, 26695, and G27, gene CAD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.195 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Helicobacter pylori

Storage Stability

EC Number Storage Stability Organism
1.1.1.195 -20°C, purified recombinant His-tagged enzyme, 75 mM sodium phosphate, pH 7.5, 5 mM DTT, no loss of activity after 1 month Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 benzaldehyde + NADP+ the enzyme also shows dismutase activity Helicobacter pylori benzoic acid + NADPH
-
 ?
1.1.1.195 benzyl alcohol + NADP+
-
 Helicobacter pylori benzaldehyde + NADPH
-
 r
1.1.1.195 butanol + NADP+
-
 Helicobacter pylori butyraldehyde + NADPH
-
 r
1.1.1.195 cinnamyl alcohol + NADP+
-
 Helicobacter pylori cinnamyl aldehyde + NADPH
-
 r
1.1.1.195 coniferyl alcohol + NADP+
-
 Helicobacter pylori coniferyl aldehyde + NADPH + H+
-
 r
1.1.1.195 ethanol + NADP+
-
 Helicobacter pylori acetaldehyde + NADPH + H+
-
 r
1.1.1.195 propanol + NADP+
-
 Helicobacter pylori propionaldehyde + NADPH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 CAD
-
 Helicobacter pylori
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.195 37
-
 assay at Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.195 2.3
-
 benzaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 2.5
-
 benzaldehyde pH 7.5, 37°C, recombinant enzyme, dismutase reaction with NADP+ Helicobacter pylori
1.1.1.195 3.5
-
 coniferyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 5.7
-
 butanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 7.1
-
 ethanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 7.7
-
 cinnamyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 8.4
-
 benzyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 12.8
-
 Propanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 13.3
-
 cinnamyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 15.5
-
 NADP+ pH 7.5, 37°C, recombinant enzyme, with benzyla alcohol Helicobacter pylori
1.1.1.195 16.7
-
 acetaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
1.1.1.195 25.2
-
 NADPH pH 7.5, 37°C, recombinant enzyme, with acetaldehyde Helicobacter pylori
1.1.1.195 27.4
-
 coniferyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.195 7.5
-
 assay at Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADP+
-
 Helicobacter pylori
1.1.1.195 NADPH
-
 Helicobacter pylori