Literature summary extracted from

Hershfield, J.R.; Madhavarao, C.N.; Moffett, J.R.; Benjamins, J.A.; Garbern, J.Y.; Namboodiri, A.
Aspartoacylase is a regulated nuclear-cytoplasmic enzyme (2006), FASEB J., 20, 2139-2141.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.1.15	expression of the GFP-tagged enzyme in COS-7 cells in cytoplasm, nucleoplasm, and nuclei	Rattus norvegicus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.5.1.15	cytoplasm	-	Rattus norvegicus	5737	-
3.5.1.15	additional information	subcellular localization analysis, the enzyme is active in cytoplasm and in nucleus	Rattus norvegicus	-	-
3.5.1.15	nucleoplasm	-	Rattus norvegicus	5654	-
3.5.1.15	nucleus	-	Rattus norvegicus	5634	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.1.15	36000	-	about, gel filtration	Rattus norvegicus
3.5.1.15	38000	-	1 * 38000, SDS-PAGE	Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.5.1.15	N-acetylaspartic acid + H2O	Rattus norvegicus	-	L-asparatate + acetate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.15	Rattus norvegicus	-	Sprague-Dawley rat pups	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.15	native enzyme from brain by subcellular fractionation, and anion exchange chromatography	Rattus norvegicus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.5.1.15	brain	-	Rattus norvegicus	-
3.5.1.15	kidney	abundant expression, especially in proximal tubules	Rattus norvegicus	-
3.5.1.15	additional information	immunocolorimetric analysis of enzyme distribution in the brain, enzyme staining does not colocalize with CC1	Rattus norvegicus	-
3.5.1.15	oligodendrocyte	high expression level	Rattus norvegicus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.15	N-acetylaspartic acid + H2O	-	Rattus norvegicus	L-asparatate + acetate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.5.1.15	monomer	1 * 38000, SDS-PAGE	Rattus norvegicus

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.1.15	ASPA	-	Rattus norvegicus

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Release 2023.1 (January 2023)