Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kazuoka, T.; Oikawa, T.; Muraoka, I.; Kuroda, S.; Soda, K.
    A cold-active and thermostable alcohol dehydrogenase of a psychrotorelant from Antarctic seawater, Flavobacterium frigidimaris KUC-1 (2007), Extremophiles, 11, 257-267.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1
-
 Flavobacterium frigidimaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 1,10-phenanthroline 1 mM, 38% inhibition Flavobacterium frigidimaris
1.1.1.1 CuCl2 1 mM, 68% inhibition Flavobacterium frigidimaris
1.1.1.1 FeCl2 1 mM, 57% inhibition Flavobacterium frigidimaris
1.1.1.1 hexadecyltrimethyl-ammonium bromide 1 mM, 79% inhibition Flavobacterium frigidimaris
1.1.1.1 HgCl2 1 mM, complete inhibition Flavobacterium frigidimaris
1.1.1.1 Hydroxylamine hydrochloride 1 mM, 34% inhibition Flavobacterium frigidimaris
1.1.1.1 iodoacetic acid 1 mM, 96% inhibition Flavobacterium frigidimaris
1.1.1.1 NEM 1 mM, complete inhibition Flavobacterium frigidimaris
1.1.1.1 NiCl2 1 mM, 79% inhibition Flavobacterium frigidimaris
1.1.1.1 PCMB 1 mM, complete inhibition Flavobacterium frigidimaris
1.1.1.1 polyoxyethylene octylphenyl ether 1 mM, 43% inhibition Flavobacterium frigidimaris
1.1.1.1 SDS 1 mM, 91% inhibition Flavobacterium frigidimaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.147
-
 ethanol 20°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 0.168
-
 ethanol 30°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 0.19
-
 ethanol 10°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 0.248
-
 ethanol 40°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 0.43
-
 ethanol 50°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 1.04
-
 ethanol 60°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 5.7
-
 ethanol 20°C, pH 9.0 Saccharomyces cerevisiae
1.1.1.1 8.56
-
 ethanol 30°C, pH 9.0 Saccharomyces cerevisiae
1.1.1.1 9.03
-
 ethanol 10°C, pH 9.0 Saccharomyces cerevisiae
1.1.1.1 12.1
-
 ethanol 40°C, pH 9.0 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zn the enzyme contains 2 gram-atoms Zn per subunit Flavobacterium frigidimaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 40000
-
 4 * 40000, SDS-PAGE Flavobacterium frigidimaris
1.1.1.1 160000
-
 gel filtration Flavobacterium frigidimaris

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Flavobacterium frigidimaris Q8L3C9
-
-
1.1.1.1 Flavobacterium frigidimaris KUC-1 Q8L3C9
-
-
1.1.1.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Flavobacterium frigidimaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 43
-
-
 Flavobacterium frigidimaris

Storage Stability

EC Number Storage Stability Organism
1.1.1.1 -20°C, 10 mM potassium phosphate buffer, pH 7.0, containing 0.5 mM NAD+ without loss of activity for several months Flavobacterium frigidimaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-butanol + NAD+
-
 Flavobacterium frigidimaris butanal + NADH + H+
-
 r
1.1.1.1 1-butanol + NAD+
-
 Flavobacterium frigidimaris KUC-1 butanal + NADH + H+
-
 r
1.1.1.1 1-decanol + NAD+
-
 Flavobacterium frigidimaris decanal + NADH + H+
-
 r
1.1.1.1 1-decanol + NAD+
-
 Flavobacterium frigidimaris KUC-1 decanal + NADH + H+
-
 r
1.1.1.1 1-heptanol + NAD+
-
 Flavobacterium frigidimaris heptanal + NADH + H+
-
 r
1.1.1.1 1-heptanol + NAD+
-
 Flavobacterium frigidimaris KUC-1 heptanal + NADH + H+
-
 r
1.1.1.1 1-hexanol + NAD+
-
 Flavobacterium frigidimaris hexanal + NADH + H+
-
 r
1.1.1.1 1-nonanol + NAD+
-
 Flavobacterium frigidimaris nonanal + NADH + H+
-
 r
1.1.1.1 1-octanol + NAD+
-
 Flavobacterium frigidimaris octanal + NADH + H+
-
 r
1.1.1.1 1-pentanol + NAD+
-
 Flavobacterium frigidimaris pentanal + NADH + H+
-
 r
1.1.1.1 1-propanol + NAD+
-
 Flavobacterium frigidimaris propanal + NADH + H+
-
 r
1.1.1.1 2-butanol + NAD+ weak activity Flavobacterium frigidimaris 2-butanone + NADH + H+
-
 r
1.1.1.1 2-decanol + NAD+ weak activity Flavobacterium frigidimaris 2-decanone + NADH + H+
-
 r
1.1.1.1 2-heptanol + NAD+ weak activity Flavobacterium frigidimaris 2-heptanone + NADH + H+
-
 r
1.1.1.1 2-hexanol + NAD+ weak activity Flavobacterium frigidimaris 2-hexanone + NADH + H+
-
 r
1.1.1.1 2-nonanol + NAD+ weak activity Flavobacterium frigidimaris 2-nonanone + NADH + H+
-
 r
1.1.1.1 2-octanol + NAD+ weak activity Flavobacterium frigidimaris 2-octanone + NADH + H+
-
 r
1.1.1.1 2-pentanol + NAD+ weak activity Flavobacterium frigidimaris 2-pentanone + NADH + H+
-
 r
1.1.1.1 2-propanol + NAD+
-
 Flavobacterium frigidimaris acetone + NADH + H+
-
 r
1.1.1.1 2-propanol + NAD+
-
 Flavobacterium frigidimaris KUC-1 acetone + NADH + H+
-
 r
1.1.1.1 allylalcohol + NAD+
-
 Flavobacterium frigidimaris prop-2-enal + NADH + H+
-
 ?
1.1.1.1 benzylalcohol + NAD+ weak activity Flavobacterium frigidimaris benzaldehyde + NADH + H+
-
 r
1.1.1.1 ethanol + NAD+
-
 Saccharomyces cerevisiae acetaldehyde + NADH + H+
-
 r
1.1.1.1 ethanol + NAD+
-
 Flavobacterium frigidimaris acetaldehyde + NADH + H+
-
 r
1.1.1.1 additional information no activity with methanol Flavobacterium frigidimaris ?
-
 ?
1.1.1.1 additional information no activity with methanol Flavobacterium frigidimaris KUC-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 tetramer 4 * 40000, SDS-PAGE Flavobacterium frigidimaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 30
-
-
 Saccharomyces cerevisiae
1.1.1.1 70
-
-
 Flavobacterium frigidimaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1
-
 60 0°C: about 70% of maximal activity, about 45% of maximal activity Flavobacterium frigidimaris
1.1.1.1 20 85 20°C: about 65% of maximal activity, 85°C: about 90% of maximal activity Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 50
-
 half-life: 304 min in absence of NAD+ Flavobacterium frigidimaris
1.1.1.1 60
-
 half-life: 2 min in absence of NAD+ Saccharomyces cerevisiae
1.1.1.1 60
-
 half-life: 50 min in absence of NAD+, 143 min in presence of 0.01 mM NAD+ Flavobacterium frigidimaris
1.1.1.1 65
-
 half-life: 20 min in absence of NAD+ Flavobacterium frigidimaris
1.1.1.1 70
-
 half-life: 6 min in absence of NAD+ Flavobacterium frigidimaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 39.9
-
 ethanol 10°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 62.48
-
 ethanol 20°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 109.5
-
 ethanol 30°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 160.8
-
 ethanol 40°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 251.3
-
 ethanol 10°C, pH 9.0 Saccharomyces cerevisiae
1.1.1.1 262.3
-
 ethanol 50°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 432.3
-
 ethanol 20°C, pH 9.0 Saccharomyces cerevisiae
1.1.1.1 474.9
-
 ethanol 60°C, pH 9.0 Flavobacterium frigidimaris
1.1.1.1 786.1
-
 ethanol 30°C, pH 9.0 Saccharomyces cerevisiae
1.1.1.1 895.4
-
 ethanol 40°C, pH 9.0 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6
-
 reduction of acetaldehyde Flavobacterium frigidimaris
1.1.1.1 6.7 7 oxidation of ethanol Flavobacterium frigidimaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ specific for NAD+, no activity with NADP+, pro-R stereospecificity for hydrogen transfer Flavobacterium frigidimaris