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Literature summary extracted from

  • Topakas, E.; Stamatis, H.; Mastihubova, M.; Biely, P.; Kekos, D.; Macris, B.J.; Christakopoulos, P.
    Purification and characterization of a Fusarium oxysporum feruloyl esterase (FoFAE-I) catalysing transesterification of phenolic acid esters (2003), Enzyme Microb. Technol., 33, 729-737.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.73 synthesis ferulic, p-coumaric, caffeic, and sinapinic acid have widespread industrial potential due to their antioxidant properties Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.06
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 0.2
-
 methyl p-coumarate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 0.26
-
 methyl caffeate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 0.6
-
 methyl ferulate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 1.12
-
 methyl sinapinate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 1.6
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside pH 6.0, 45°C Fusarium oxysporum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Fusarium oxysporum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 30000
-
 gel filtration Fusarium oxysporum
3.1.1.73 31000
-
 1 * 31000, SDS-PAGE Fusarium oxysporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 destarched wheat bran + H2O Fusarium oxysporum most effective in the presence of added xylanase ferulic acid + ?
-
 ?
3.1.1.73 destarched wheat bran + H2O Fusarium oxysporum F3 most effective in the presence of added xylanase ferulic acid + ?
-
 ?
3.1.1.73 plant cell wall + H2O Fusarium oxysporum involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 plant cell wall + H2O Fusarium oxysporum F3 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.73 Butanol transesterifiaction in microemulsion of hexane/1-butanol/water Fusarium oxysporum
3.1.1.73 hexane transesterifiaction in microemulsion of hexane/1-butanol/water Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Fusarium oxysporum
-
-
-
3.1.1.73 Fusarium oxysporum F3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Fusarium oxysporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 6.94
-
 purified enzyme, pH 6.0, 45°C Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O C-2 position is hydrolyzed faster and more effectively than C-5 position Fusarium oxysporum ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
3.1.1.73 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O C-2 position is hydrolyzed faster and more effectively than C-5 position Fusarium oxysporum F3 ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
3.1.1.73 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O C-2 position is hydrolyzed faster and more effectively than C-5 position Fusarium oxysporum ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
3.1.1.73 destarched wheat bran + H2O most effective in the presence of added xylanase Fusarium oxysporum ferulic acid + ?
-
 ?
3.1.1.73 destarched wheat bran + H2O most effective in the presence of added xylanase Fusarium oxysporum F3 ferulic acid + ?
-
 ?
3.1.1.73 methyl caffeate + 1-butanol transesterifiaction in microemulsion of hexane/1-butanol/water Fusarium oxysporum methanol + butyl caffeate
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Fusarium oxysporum caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Fusarium oxysporum F3 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + 1-butanol transesterifiaction in microemulsion of hexane/1-butanol/water Fusarium oxysporum methanol + butyl ferulate
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Fusarium oxysporum ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + 1-butanol transesterifiaction in microemulsion of hexane/1-butanol/water Fusarium oxysporum methanol + butyl p-coumarate
-
 ?
3.1.1.73 methyl p-coumarate + H2O most effective substrate Fusarium oxysporum p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + 1-butanol transesterifiaction in microemulsion of hexane/1-butanol/water Fusarium oxysporum methanol + butyl sinapinate
-
 ?
3.1.1.73 methyl sinapinate + H2O poor substrate Fusarium oxysporum sinapic acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O poor substrate Fusarium oxysporum F3 sinapic acid + methanol
-
 ?
3.1.1.73 plant cell wall + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Fusarium oxysporum ferulic acid + ?
-
 ?
3.1.1.73 plant cell wall + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Fusarium oxysporum F3 ferulic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 31000, SDS-PAGE Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FoFAE-I
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 55
-
-
 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 30
-
 fully stable at pH 7.0-9.0 Fusarium oxysporum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.5
-
 methyl sinapinate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 5.9
-
 methyl caffeate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 6.9
-
 methyl ferulate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 19.9
-
 methyl p-coumarate pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 166.7
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside pH 6.0, 45°C Fusarium oxysporum
3.1.1.73 600
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside pH 6.0, 45°C Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
-
 Fusarium oxysporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 7 9 fully stable at 30°C Fusarium oxysporum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Fusarium oxysporum isoelectric focusing
-
 9.5