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Literature summary extracted from

  • Beriault, R.; Chenier, D.; Singh, R.; Middaugh, J.; Mailloux, R.; Appanna, V.
    Detection and purification of glucose 6-phosphate dehydrogenase, malic enzyme, and NADP-dependent isocitrate dehydrogenase by blue native polyacrylamide gel electrophoresis (2005), Electrophoresis, 26, 2892-2897.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.42 analysis simultaneous detection, quantitation and purification of glucose 6-phosphate dehydrogenase, malic enzyme, and NADP-dependent isocitrate dehydrogenase by blue native gel electrophoresis Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+ Pseudomonas fluorescens
-
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?
1.1.1.49 D-glucose 6-phosphate + NADP+ Pseudomonas fluorescens 13525
-
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Pseudomonas fluorescens
-
-
-
1.1.1.49 Pseudomonas fluorescens
-
-
-
1.1.1.49 Pseudomonas fluorescens 13525
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 native enzyme by blue native PAGE to homogeneity, method optimization, overview Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.49 additional information
-
in-gel staining of purified native enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+
-
Pseudomonas fluorescens D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?
1.1.1.49 D-glucose 6-phosphate + NADP+
-
Pseudomonas fluorescens 13525 D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 G6PDH
-
Pseudomonas fluorescens
1.1.1.49 glucose 6-phosphate dehydrogenase
-
Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
Pseudomonas fluorescens