EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.30 | expression in Escherichia coli | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.30 | H141A | kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 12.9fold lower than wild-type enzyme | Pseudomonas putida |
1.1.1.30 | K149A | inactive mutant enzyme | Pseudomonas putida |
1.1.1.30 | K149R | kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 7.2fold lower than wild-type enzyme | Pseudomonas putida |
1.1.1.30 | Q193A | kcat/KM for (R)-3-hydroxybutanoate is 307fold lower than wild-type value, kcat/Km for NAD+ is 6.2fold lower than wild-type enzyme | Pseudomonas putida |
1.1.1.30 | Q91A | kcat/KM for (R)-3-hydroxybutanoate is 83.7fold lower than wild-type value, kcat/Km for NAD+ is 2.8fold lower than wild-type enzyme | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.30 | 0.18 | - |
NAD+ | 23°C, pH 8.0, wild-type enzyme | Pseudomonas putida | |
1.1.1.30 | 0.27 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme Q193A | Pseudomonas putida | |
1.1.1.30 | 0.28 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme H141A | Pseudomonas putida | |
1.1.1.30 | 0.38 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme K149R | Pseudomonas putida | |
1.1.1.30 | 0.51 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme Q91A | Pseudomonas putida | |
1.1.1.30 | 1.5 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, wild-type enzyme | Pseudomonas putida | |
1.1.1.30 | 35 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme H141A | Pseudomonas putida | |
1.1.1.30 | 78 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme K149R | Pseudomonas putida | |
1.1.1.30 | 96 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q193A | Pseudomonas putida | |
1.1.1.30 | 131 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q91A | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.30 | Pseudomonas putida | Q9AE70 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.30 | - |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.30 | (R)-3-hydroxybutanoate + NAD+ | - |
Pseudomonas putida | acetoacetate + NADH + H+ | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.30 | 167 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme H141A | Pseudomonas putida | |
1.1.1.30 | 333 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q193A | Pseudomonas putida | |
1.1.1.30 | 404 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme K149R | Pseudomonas putida | |
1.1.1.30 | 1382 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, wild-type enzyme | Pseudomonas putida | |
1.1.1.30 | 1390 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q91A | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.30 | NAD+ | - |
Pseudomonas putida |