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Literature summary extracted from

  • Feller, C.; Guenther, R.; Hofmann, H.J.; Grunow, M.
    Molecular basis of substrate recognition in D-3-hydroxybutyrate dehydrogenase from Pseudomonas putida (2006), ChemBioChem, 7, 1410-1418.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.30 expression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.30 H141A kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 12.9fold lower than wild-type enzyme Pseudomonas putida
1.1.1.30 K149A inactive mutant enzyme Pseudomonas putida
1.1.1.30 K149R kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 7.2fold lower than wild-type enzyme Pseudomonas putida
1.1.1.30 Q193A kcat/KM for (R)-3-hydroxybutanoate is 307fold lower than wild-type value, kcat/Km for NAD+ is 6.2fold lower than wild-type enzyme Pseudomonas putida
1.1.1.30 Q91A kcat/KM for (R)-3-hydroxybutanoate is 83.7fold lower than wild-type value, kcat/Km for NAD+ is 2.8fold lower than wild-type enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.18
-
NAD+ 23°C, pH 8.0, wild-type enzyme Pseudomonas putida
1.1.1.30 0.27
-
NAD+ 23°C, pH 8.0, mutant enzyme Q193A Pseudomonas putida
1.1.1.30 0.28
-
NAD+ 23°C, pH 8.0, mutant enzyme H141A Pseudomonas putida
1.1.1.30 0.38
-
NAD+ 23°C, pH 8.0, mutant enzyme K149R Pseudomonas putida
1.1.1.30 0.51
-
NAD+ 23°C, pH 8.0, mutant enzyme Q91A Pseudomonas putida
1.1.1.30 1.5
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, wild-type enzyme Pseudomonas putida
1.1.1.30 35
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme H141A Pseudomonas putida
1.1.1.30 78
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme K149R Pseudomonas putida
1.1.1.30 96
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q193A Pseudomonas putida
1.1.1.30 131
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q91A Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Pseudomonas putida Q9AE70
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30
-
Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
Pseudomonas putida acetoacetate + NADH + H+
-
r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.30 167
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme H141A Pseudomonas putida
1.1.1.30 333
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q193A Pseudomonas putida
1.1.1.30 404
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme K149R Pseudomonas putida
1.1.1.30 1382
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, wild-type enzyme Pseudomonas putida
1.1.1.30 1390
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q91A Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
Pseudomonas putida