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Literature summary extracted from

  • de Groot, M.J.; Prathumpai, W.; Visser, J.; Ruijter, G.J.
    Metabolic control analysis of Aspergillus niger L-arabinose catabolism (2005), Biotechnol. Prog., 21, 1610-1616.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.12 0.008
-
 NADH 30°C, pH 9.6 Aspergillus niger
1.1.1.12 0.05
-
 NAD+ 30°C, pH 9.6 Aspergillus niger
1.1.1.12 5
-
 L-xylulose 30°C, pH 9.6 Aspergillus niger
1.1.1.12 8 9 L-arabinitol 30°C, pH 9.6 Aspergillus niger
1.1.1.431 0.008
-
 NADPH 30°C, cosubstrate: D-xylose or L-arabinose Aspergillus niger
1.1.1.431 39
-
 D-xylose 30°C Aspergillus niger
1.1.1.431 93
-
 L-arabinose 30°C Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.12 L-arabinitol + NAD+ Aspergillus niger enzyme of the L-arabinose catabolic pathway L-xylulose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.12 Aspergillus niger
-
-
-
1.1.1.431 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.12 partial Aspergillus niger
1.1.1.431
-
 Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.431 mycelium
-
 Aspergillus niger
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.12 -70°C, stable during storage for at least 2 years Aspergillus niger
1.1.1.12 4°C, stable during storage for months Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.12 L-arabinitol + NAD+
-
 Aspergillus niger L-xylulose + NADH + H+
-
 r
1.1.1.12 L-arabinitol + NAD+ enzyme of the L-arabinose catabolic pathway Aspergillus niger L-xylulose + NADH + H+
-
 r
1.1.1.431 D-xylose + NADPH + H+
-
 Aspergillus niger xylitol + NADP+
-
 ?
1.1.1.431 L-arabinose + NADPH + H+
-
 Aspergillus niger L-arabitol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.431 D-xylose reductase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.431 30
-
 assay at Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.12 NAD+
-
 Aspergillus niger
1.1.1.12 NADH
-
 Aspergillus niger
1.1.1.431 NADPH
-
 Aspergillus niger