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Literature summary extracted from

  • Tazoe, M.; Ichikawa, K.; Hoshino, T.
    Purification and characterization of pyridoxine 5'-phosphate phosphatase from Sinorhizobium meliloti (2005), Biosci. Biotechnol. Biochem., 69, 2277-2284.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.74 iodacetate 52% inhibition at 5 mM Sinorhizobium meliloti
3.1.3.74 KH2PO4 56% inhibition at 5 mM Sinorhizobium meliloti
3.1.3.74 levamisole 26% inhibition at 5 mM Sinorhizobium meliloti
3.1.3.74 N-ethylmaleimide 46% inhibition at 5 mM Sinorhizobium meliloti
3.1.3.74 NaF 24% inhibition at 5 mM Sinorhizobium meliloti
3.1.3.74 Sodium molybdate 79% inhibition at 5 mM Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.74 0.385
-
pyridoxal-5'-phosphate 1 mM MnCl2, 50 mM Tris-HCl pH 7.0 Sinorhizobium meliloti
3.1.3.74 0.385
-
pyridoxine-5'-phosphate 1 mM MnCl2, 50 mM Tris-HCl pH 7.0 Sinorhizobium meliloti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.74 cytoplasm
-
Sinorhizobium meliloti 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.74 Mg2+ either Mg2+ or Mn2+ required, highest activity at 2 mM Sinorhizobium meliloti
3.1.3.74 Mn2+ either Mg2+ or Mn2+ required, highest activity at 2 mM Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.74 29000
-
gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.74 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.74 5 chromatography steps to homogeneity Sinorhizobium meliloti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.74 cell culture
-
Sinorhizobium meliloti
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.74 42
-
1 mM MnCl2, 50 mM Tris-HCl pH 7.0 Sinorhizobium meliloti
3.1.3.74 105
-
1 mM MnCl2, 50 mM Tris-HCl pH 7.0 Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.74 pyridoxal-5'-phosphate + H2O
-
Sinorhizobium meliloti pyridoxal + phosphate
-
?
3.1.3.74 pyridoxine-5'-phosphate + H2O
-
Sinorhizobium meliloti pyridoxine + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.74 monomer 1 * 29000, SDS-PAGE Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
3.1.3.74 PNP phosphatase
-
Sinorhizobium meliloti
3.1.3.74 pyridoxal-5'-phosphate phosphatase
-
Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.74 7.5
-
-
Sinorhizobium meliloti

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.74 5.5 9.5
-
Sinorhizobium meliloti