EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.74 | iodacetate | 52% inhibition at 5 mM | Sinorhizobium meliloti | |
3.1.3.74 | KH2PO4 | 56% inhibition at 5 mM | Sinorhizobium meliloti | |
3.1.3.74 | levamisole | 26% inhibition at 5 mM | Sinorhizobium meliloti | |
3.1.3.74 | N-ethylmaleimide | 46% inhibition at 5 mM | Sinorhizobium meliloti | |
3.1.3.74 | NaF | 24% inhibition at 5 mM | Sinorhizobium meliloti | |
3.1.3.74 | Sodium molybdate | 79% inhibition at 5 mM | Sinorhizobium meliloti |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.74 | 0.385 | - |
pyridoxal-5'-phosphate | 1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti | |
3.1.3.74 | 0.385 | - |
pyridoxine-5'-phosphate | 1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.74 | cytoplasm | - |
Sinorhizobium meliloti | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.74 | Mg2+ | either Mg2+ or Mn2+ required, highest activity at 2 mM | Sinorhizobium meliloti | |
3.1.3.74 | Mn2+ | either Mg2+ or Mn2+ required, highest activity at 2 mM | Sinorhizobium meliloti |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 29000 | - |
gel filtration | Sinorhizobium meliloti |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.74 | Sinorhizobium meliloti | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.74 | 5 chromatography steps to homogeneity | Sinorhizobium meliloti |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.74 | cell culture | - |
Sinorhizobium meliloti | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 42 | - |
1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti |
3.1.3.74 | 105 | - |
1 mM MnCl2, 50 mM Tris-HCl pH 7.0 | Sinorhizobium meliloti |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.74 | pyridoxal-5'-phosphate + H2O | - |
Sinorhizobium meliloti | pyridoxal + phosphate | - |
? | |
3.1.3.74 | pyridoxine-5'-phosphate + H2O | - |
Sinorhizobium meliloti | pyridoxine + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.74 | monomer | 1 * 29000, SDS-PAGE | Sinorhizobium meliloti |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.74 | PNP phosphatase | - |
Sinorhizobium meliloti |
3.1.3.74 | pyridoxal-5'-phosphate phosphatase | - |
Sinorhizobium meliloti |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 7.5 | - |
- |
Sinorhizobium meliloti |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.74 | 5.5 | 9.5 | - |
Sinorhizobium meliloti |