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Literature summary extracted from

  • Takao, S.; Ito, T.; Tanida, M.
    Purification and kinetic properties of butyryl-CoA synthetase from Paecilomyces varioti (1987), Agric. Biol. Chem., 51, 145-152.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.2 AMP competitive for ATP Paecilomyces variotii
6.2.1.2 butyryl-CoA competitive for ATP Paecilomyces variotii
6.2.1.2 diphosphate competitive for CoA Paecilomyces variotii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.2 0.13
-
 CoA
-
 Paecilomyces variotii
6.2.1.2 0.14
-
 Butyrate ATP Paecilomyces variotii
6.2.1.2 0.38
-
 pentanoate
-
 Paecilomyces variotii
6.2.1.2 0.47
-
 Isopentanoate
-
 Paecilomyces variotii
6.2.1.2 1.6
-
 Isobutyrate
-
 Paecilomyces variotii
6.2.1.2 3.3
-
 propionate
-
 Paecilomyces variotii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.2 Ca2+ can replace Mg2+ in activation with 47% of the efficiency, Km: 0.14 mM Paecilomyces variotii
6.2.1.2 K+ requires both Mg2+ and K+, Km: 2.4 mM Paecilomyces variotii
6.2.1.2 Mg2+ requires both Mg2+ and K+ Paecilomyces variotii
6.2.1.2 Mn2+ can replace Mg2+ in activation Paecilomyces variotii
6.2.1.2 Mn2+ with 128% of the activation compared to Mg2+, Km: 0.14 mM Paecilomyces variotii
6.2.1.2 Rb+ can replace K+ in activation with 81% efficiency, Km: 2.4 mM Paecilomyces variotii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.2 74000
-
 2 * 74000, SDS-PAGE Paecilomyces variotii
6.2.1.2 145000
-
 gel filtration Paecilomyces variotii

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.2 Paecilomyces variotii
-
-
-
6.2.1.2 Paecilomyces variotii AHU 9417
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.2
-
 Paecilomyces variotii

Reaction

EC Number Reaction Comment Organism Reaction ID
6.2.1.2 ATP + a medium-chain fatty acid + CoA = AMP + diphosphate + a medium-chain acyl-CoA bi uni uni bi ping-pong mechanism, in which the order of addition and evolution of substrates and products is ATP, butyrate, diphosphate, CoA, butyryl-CoA or AMP, and AMP or butyryl-CoA. Butyryl-CoA and AMP are evolved at random or at the same time Paecilomyces variotii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.2 additional information
-
-
 Paecilomyces variotii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.2 ATP + butyrate + CoA
-
 Paecilomyces variotii AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.2 ATP + butyrate + CoA
-
 Paecilomyces variotii AHU 9417 AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.2 ATP + isobutyrate + CoA
-
 Paecilomyces variotii AMP + diphosphate + isobutyryl-CoA
-
 ?
6.2.1.2 ATP + isopentanoate
-
 Paecilomyces variotii AMP + diphosphate + isopentanoyl-CoA
-
 ?
6.2.1.2 ATP + pentanoate + CoA
-
 Paecilomyces variotii AMP + diphosphate + pentanoyl-CoA
-
 ?
6.2.1.2 ATP + pentanoate + CoA
-
 Paecilomyces variotii AHU 9417 AMP + diphosphate + pentanoyl-CoA
-
 ?
6.2.1.2 ATP + propionate + CoA
-
 Paecilomyces variotii AMP + diphosphate + propionyl-CoA
-
 ?
6.2.1.2 ATP + propionate + CoA
-
 Paecilomyces variotii AHU 9417 AMP + diphosphate + propionyl-CoA
-
 ?
6.2.1.2 additional information no activity with hexanoate Paecilomyces variotii ?
-
 ?
6.2.1.2 additional information no activity with acetate and octanoate Paecilomyces variotii ?
-
 ?
6.2.1.2 additional information no activity with hexanoate Paecilomyces variotii AHU 9417 ?
-
 ?
6.2.1.2 additional information no activity with acetate and octanoate Paecilomyces variotii AHU 9417 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.2 dimer 2 * 74000, SDS-PAGE Paecilomyces variotii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.2 35
-
-
 Paecilomyces variotii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.2 9
-
-
 Paecilomyces variotii