EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.77 | expression in Escherichia coli | Ensifer adhaerens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.77 | Ag+ | 2 mM, 97.6% inhibition | Ensifer adhaerens | |
3.5.1.77 | Ba2+ | 2 mM, 8% inhibition | Ensifer adhaerens | |
3.5.1.77 | Co2+ | 2 mM, 67% inhibition | Ensifer adhaerens | |
3.5.1.77 | Cu2+ | 2 mM, 98.4% inhibition | Ensifer adhaerens | |
3.5.1.77 | H2O2 | 32% loss of activity after treatment with 0.1 mM H2O2 for 30 min, 57% loss of activity after treatment with 0.2 mM H2O2 for 30 min | Ensifer adhaerens | |
3.5.1.77 | Mg2+ | 2 mM, 24.6% inhibition | Ensifer adhaerens | |
3.5.1.77 | Mn2+ | 2 mM, 58% inhibition | Ensifer adhaerens | |
3.5.1.77 | Sn2+ | 2 mM, 46.5% inhibition | Ensifer adhaerens | |
3.5.1.77 | Zn2+ | 2 mM, 98% inhibition | Ensifer adhaerens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.77 | 3.76 | - |
N-carbamoyl-D-p-hydroxyphenylglycine | pH 7.0, 40°C | Ensifer adhaerens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.77 | Ca2+ | 2 mM, enhances activity to about 110% | Ensifer adhaerens | |
3.5.1.77 | Fe2+ | 2 mM, enhances activity to about 110% | Ensifer adhaerens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 34363 | - |
4 * 34363, calculated from sequence | Ensifer adhaerens |
3.5.1.77 | 38000 | - |
4 * 38000, SDS-PAGE | Ensifer adhaerens |
3.5.1.77 | 150000 | - |
gel filtration | Ensifer adhaerens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.77 | Ensifer adhaerens | Q5S260 | - |
- |
3.5.1.77 | Ensifer adhaerens S-5 | Q5S260 | - |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
3.5.1.77 | high oxidative stability | Ensifer adhaerens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.77 | recombinant enzyme | Ensifer adhaerens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 9.25 | - |
enzyme from Sinorhizobium morelens | Ensifer adhaerens |
3.5.1.77 | 21.4 | - |
enzyme recombinantly expressed in Escherichia coli | Ensifer adhaerens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.77 | additional information | strict specificity towards N-carbamoyl-D-amino acids, no activity with N-carbamoyl-L-amino acids | Ensifer adhaerens | ? | - |
? | |
3.5.1.77 | additional information | strict specificity towards N-carbamoyl-D-amino acids, no activity with N-carbamoyl-L-amino acids | Ensifer adhaerens S-5 | ? | - |
? | |
3.5.1.77 | N-carbamoyl-D-Ala + H2O | 13% of activity with N-carbamoyl-D-p-hydroxyphenylglycine | Ensifer adhaerens | D-Ala + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-Ala + H2O | 13% of activity with N-carbamoyl-D-p-hydroxyphenylglycine | Ensifer adhaerens S-5 | D-Ala + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-Leu + H2O | 43% of activity with N-carbamoyl-D-p-hydroxyphenylglycine | Ensifer adhaerens | D-Leu + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-Leu + H2O | 43% of activity with N-carbamoyl-D-p-hydroxyphenylglycine | Ensifer adhaerens S-5 | D-Leu + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-Met + H2O | 48% of activity with N-carbamoyl-D-p-hydroxyphenylglycine | Ensifer adhaerens | D-Met + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-p-hydroxyphenylglycine + H2O | - |
Ensifer adhaerens | D-p-hydroxyphenylglycine + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-p-hydroxyphenylglycine + H2O | - |
Ensifer adhaerens S-5 | D-p-hydroxyphenylglycine + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-phenylglycine + H2O | 58% of activity with N-carbamoyl-D-p-hydroxyphenylglycine | Ensifer adhaerens | D-phenylglycine + NH3 + CO2 | - |
? | |
3.5.1.77 | N-carbamoyl-D-phenylglycine + H2O | 58% of activity with N-carbamoyl-D-p-hydroxyphenylglycine | Ensifer adhaerens S-5 | D-phenylglycine + NH3 + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.77 | tetramer | 4 * 38000, SDS-PAGE | Ensifer adhaerens |
3.5.1.77 | tetramer | 4 * 34363, calculated from sequence | Ensifer adhaerens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 60 | - |
- |
Ensifer adhaerens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 45 | 65 | 45°C: about 50% of maximal activity, 65°C: about 75% of maximal activity | Ensifer adhaerens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 50 | - |
pH 7.0, 30 min, stable up to | Ensifer adhaerens |
3.5.1.77 | 55 | - |
pH 7.0, 30 min, 10% loss of activity | Ensifer adhaerens |
3.5.1.77 | 60 | - |
pH 7.0, 30 min, about 50% loss of activity | Ensifer adhaerens |
3.5.1.77 | 65 | - |
pH 7.0, 30 min, about 90% of maximal activity | Ensifer adhaerens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 7 | - |
- |
Ensifer adhaerens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 6.5 | 7.5 | pH 6.5: about 90% of maximal activity, pH 7.5: about 95% of maximal activity | Ensifer adhaerens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.77 | 6.5 | 8.3 | 30 min, 30 min, stable | Ensifer adhaerens |