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Literature summary extracted from

  • Dabonne, S.; Moallic, C.; Sine, J.P.; Niamke, S.; Dion, M.; Colas, B.
    Cloning, expression and characterization of a 46.5-kDa metallopeptidase from Bacillus halodurans H4 sharing properties with the pitrilysin family (2005), Biochim. Biophys. Acta, 1725, 136-143.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.55
-
Halalkalibacterium halodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.55 1,10-phenanthroline 1 mM, 70% inhibition Halalkalibacterium halodurans
3.4.24.55 2-mercaptoethanol 1% w/v, 95% inhibition Halalkalibacterium halodurans
3.4.24.55 Co2+ 1 mM, complete inhibition Halalkalibacterium halodurans
3.4.24.55 Cu2+ 1 mM, complete inhibition Halalkalibacterium halodurans
3.4.24.55 cysteine 1 mM, complete inhibition Halalkalibacterium halodurans
3.4.24.55 E-64 1 mM, 46% inhibition Halalkalibacterium halodurans
3.4.24.55 EDTA 1 mM, complete inhibition Halalkalibacterium halodurans
3.4.24.55 EGTA 1 mM, complete inhibition Halalkalibacterium halodurans
3.4.24.55 Fe2+ 1 mM, 76% inhibition Halalkalibacterium halodurans
3.4.24.55 N-ethylmaleimide 1 mM, 95% inhibition Halalkalibacterium halodurans
3.4.24.55 Zn2+ 1 mM, complete inhibition Halalkalibacterium halodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.55 0.05
-
Insulin B-chain pH 8.0, 37°C Halalkalibacterium halodurans
3.4.24.55 0.12
-
dynorphin A fragment 1-13 pH 8.0, 37°C Halalkalibacterium halodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.55 Mn2+ marked activation Halalkalibacterium halodurans
3.4.24.55 Zn2+ protein contains 1 atom per molecule Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.55 46500
-
gel filtration Halalkalibacterium halodurans
3.4.24.55 46585
-
1 * 46500, SDS-PAGE, 1 * 46585, calculated Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.55 Halalkalibacterium halodurans
-
-
-
3.4.24.55 Halalkalibacterium halodurans H4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.55 recombinant protein with His-tag Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.55 dynorphin + H2O
-
Halalkalibacterium halodurans ?
-
?
3.4.24.55 dynorphin + H2O
-
Halalkalibacterium halodurans H4 ?
-
?
3.4.24.55 dynorphin A fragment 1-13 + H2O cleavage at Phe4-Leu5, Leu5-Arg6, Arg6-Arg7, Arg7-Ile8 Halalkalibacterium halodurans ?
-
?
3.4.24.55 dynorphin A fragment 1-13 + H2O cleavage at Phe4-Leu5, Leu5-Arg6, Arg6-Arg7, Arg7-Ile8 Halalkalibacterium halodurans H4 ?
-
?
3.4.24.55 Insulin B-chain + H2O cleavage at Phe25-Tyr26, Tyr16-Leu17, His10-Leu11 Halalkalibacterium halodurans ?
-
?
3.4.24.55 Insulin B-chain + H2O cleavage at Phe25-Tyr26, Tyr16-Leu17, His10-Leu11 Halalkalibacterium halodurans H4 ?
-
?
3.4.24.55 additional information no substrate: 4-nitroanilides of several amino acids tested, azocasein, bovine serum albumin Halalkalibacterium halodurans ?
-
?
3.4.24.55 additional information no substrate: 4-nitroanilides of several amino acids tested, azocasein, bovine serum albumin Halalkalibacterium halodurans H4 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.55 monomer 1 * 46500, SDS-PAGE, 1 * 46585, calculated Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
3.4.24.55 ppBH4
-
Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.55 45
-
-
Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.55 40
-
10 min, stable Halalkalibacterium halodurans
3.4.24.55 50
-
half-life is 20 min Halalkalibacterium halodurans
3.4.24.55 55
-
half-life is 10 min Halalkalibacterium halodurans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.55 0.18
-
Insulin B-chain pH 8.0, 37°C Halalkalibacterium halodurans
3.4.24.55 0.55
-
dynorphin A fragment 1-13 pH 8.0, 37°C Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.55 8
-
activity in sodium phosphate buffer is 3- to 4fold higher than in veronal or Tris/HCl buffer Halalkalibacterium halodurans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.55 Halalkalibacterium halodurans calculated
-
5.4