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Literature summary extracted from

  • Silva, R.G.; de Carvalho, L.P.; Blanchard, J.S.; Santos, D.S.; Basso, L.A.
    Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein (ACP) reductase: kinetic and chemical mechanisms (2006), Biochemistry, 45, 13064-13073.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.100 3-hydroxybutyryl-CoA noncompetitive product inhibition pattern Mycobacterium tuberculosis
1.1.1.100 NADP+ noncompetitive product inhibition pattern Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 additional information
-
 additional information detailed kinetic and thermodynamic analysis, solvent and multiple kinetic isotope effects, kinetic mechanism Mycobacterium tuberculosis
1.1.1.100 0.026
-
 NADPH pH 7.6, 30°C Mycobacterium tuberculosis
1.1.1.100 0.165
-
 acetoacetyl-CoA pH 7.6, 30°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH Mycobacterium tuberculosis
-
 3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Mycobacterium tuberculosis
-
 gene mabA
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.100 a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ chemical and kinetic mechanism Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Mycobacterium tuberculosis 3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 r
1.1.1.100 acetoacetyl-CoA + NADPH
-
 Mycobacterium tuberculosis 3-hydroxybutyryl-CoA + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 ACP reductase
-
 Mycobacterium tuberculosis
1.1.1.100 beta-ketoacyl-acyl carrier protein reductase
-
 Mycobacterium tuberculosis
1.1.1.100 MabA
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.100 25
-
 assay at Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.100 15 37 temperature profile Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.100 7
-
 acetoacetyl-CoA pH 7.0, 25°C Mycobacterium tuberculosis
1.1.1.100 7
-
 NADPH pH 7.6, 30°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.100 7
-
 assay at Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.100 5.8 10 pH profile, pH-dependence analysis, overview Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADP+
-
 Mycobacterium tuberculosis
1.1.1.100 NADPH
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.100 additional information
-
 additional information product inhibition kinetics, overview Mycobacterium tuberculosis