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Literature summary extracted from

  • Tanaka, N.; Schwer, B.
    Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22 (2005), Biochemistry, 44, 9795-9803.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.15 Poly(A) stimulates the ATP hydrolysis dependent on the chain length, highest activation by poly(A)40 comapred to shorter molecules Saccharomyces cerevisiae
3.6.1.15 RNA stimulates the ATP hydrolysis dependent on the chain length Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.15 expression of His-tagged DEAH-box Prp22 protein in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.15 additional information
-
 additional information
-
 Saccharomyces cerevisiae
3.6.1.15 0.000028
-
 ATP pH 7.5, 23°C, in presence of poly(A)40 Saccharomyces cerevisiae
3.6.1.15 0.132
-
 GTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.138
-
 ATP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.144
-
 dGTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.147
-
 dATP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.147
-
 dTTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.179
-
 dCTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.239
-
 CTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.297
-
 UTP pH 7.5, 23°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.15 Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.15 additional information Saccharomyces cerevisiae the DEAH-box splicing factor Prp22 is important in the second transesterification step of pre-mRNA splicing and is essential for release of mature mRNA from the splicosome ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.15 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.15 recombinant His-tagged DEAH-box Prp22 protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.15 ATP + H2O
-
 Saccharomyces cerevisiae ADP + phosphate
-
 ?
3.6.1.15 CTP + H2O
-
 Saccharomyces cerevisiae CDP + phosphate
-
 ?
3.6.1.15 dATP + H2O
-
 Saccharomyces cerevisiae dADP + phosphate
-
 ?
3.6.1.15 dCTP + H2O
-
 Saccharomyces cerevisiae dCDP + phosphate
-
 ?
3.6.1.15 dGTP + H2O
-
 Saccharomyces cerevisiae dGDP + phosphate
-
 ?
3.6.1.15 dTTP + H2O
-
 Saccharomyces cerevisiae dTDP + phosphate
-
 ?
3.6.1.15 GTP + H2O
-
 Saccharomyces cerevisiae GDP + phosphate
-
 ?
3.6.1.15 additional information the DEAH-box splicing factor Prp22 is important in the second transesterification step of pre-mRNA splicing and is essential for release of mature mRNA from the splicosome Saccharomyces cerevisiae ?
-
 ?
3.6.1.15 additional information DEAH-box splicing factor Prp22 shows NTPase, RNA-binding, and RNA helicase activities, ATP binding leads to dissociation of the protein from RNA, RNA binding activates the NTPase activity Saccharomyces cerevisiae ?
-
 ?
3.6.1.15 ssDNA + H2O removal of gamma-phosphate from nucleotides Saccharomyces cerevisiae ?
-
 ?
3.6.1.15 ssRNA + H2O removal of gamma-phosphate from nucleotides Saccharomyces cerevisiae ?
-
 ?
3.6.1.15 UTP + H2O
-
 Saccharomyces cerevisiae UDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.15 DEAH-box Prp22 protein
-
 Saccharomyces cerevisiae
3.6.1.15 DEAH-box splicing factor Prp22
-
 Saccharomyces cerevisiae
3.6.1.15 NTPase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.15 23
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.15 0.04 1.97 GTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.057 0.65 dATP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.5
-
 dCTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.93
-
 ATP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.97
-
 CTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 0.97
-
 UTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 9.03
-
 GTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 10.2
-
 dGTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 10.25
-
 ATP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 10.9
-
 dTTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 11.18
-
 dATP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 12.57
-
 dCTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 16.67
-
 CTP pH 7.5, 23°C Saccharomyces cerevisiae
3.6.1.15 17.28
-
 UTP pH 7.5, 23°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.15 7.5
-
 ssay at Saccharomyces cerevisiae