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Literature summary extracted from

  • Ito, R.; Hayakawa, H.; Sekiguchi, M.; Ishibashi, T.
    Multiple enzyme activities of Escherichia coli MutT protein for sanitization of DNA and RNA precursor pools (2005), Biochemistry, 44, 6670-6674.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.55 MutT protein is produced as a His-tagged form in Escherichia coli M15 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.55 0.000045
-
8-oxo-GDP pH 8.0, 30°C Escherichia coli
3.6.1.55 0.000058
-
8-oxo-dGDP pH 8.0, 30°C Escherichia coli
3.6.1.55 0.000081
-
8-oxo-dGTP pH 8.0, 30°C Escherichia coli
3.6.1.55 0.00026
-
8-oxo-GTP pH 8.0, 30°C Escherichia coli
3.6.1.55 0.17
-
dGDP pH 8.0, 30°C Escherichia coli
3.6.1.55 0.35
-
GDP pH 8.0, 30°C Escherichia coli
3.6.1.55 1
-
GTP pH 8.0, 30°C Escherichia coli
3.6.1.55 1.1
-
dGTP pH 8.0, 30°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.55 8-oxo-dGDP + H2O Escherichia coli
-
8-oxo-dGMP + phosphate
-
?
3.6.1.55 8-oxo-dGTP + H2O Escherichia coli 8-oxoguanine (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to corresponding nucleoside monophosphates, thereby preventing misincorporation of 8-oxoguanine into DNA and RNA, which would cause mutation and phenotypic suppression, respectively 8-oxo-dGMP + diphosphate
-
?
3.6.1.55 8-oxo-GDP + H2O Escherichia coli
-
8-oxo-GMP + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.55 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.55 8-oxo-dGDP + H2O
-
Escherichia coli 8-oxo-dGMP + phosphate
-
?
3.6.1.55 8-oxo-dGTP + H2O 8-oxoguanine (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to corresponding nucleoside monophosphates, thereby preventing misincorporation of 8-oxoguanine into DNA and RNA, which would cause mutation and phenotypic suppression, respectively Escherichia coli 8-oxo-dGMP + diphosphate
-
?
3.6.1.55 8-oxo-dGTP + H2O the MutT protein has an ability to cleave the phosphoanhydride bond between the alpha and beta phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates. 8-oxo-dGTP is hydrolysed with the highest efficiency Escherichia coli 8-oxo-dGMP + diphosphate
-
?
3.6.1.55 8-oxo-GDP + H2O
-
Escherichia coli 8-oxo-GMP + phosphate
-
?
3.6.1.55 8-oxo-GTP + H2O
-
Escherichia coli 8-oxo-GMP + diphosphate
-
?
3.6.1.55 dGDP + H2O
-
Escherichia coli dGMP + phosphate
-
?
3.6.1.55 dGTP + H2O
-
Escherichia coli dGMP + diphosphate
-
?
3.6.1.55 GDP + H2O
-
Escherichia coli GMP + phosphate
-
?
3.6.1.55 GTP + H2O
-
Escherichia coli GMP + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.55 MuT
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.55 30
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.55 8
-
assay at Escherichia coli