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Literature summary extracted from

  • Feng, C.; Wilson, H.L.; Tollin, G.; Astashkin, A.V.; Hazzard, J.T.; Rajagopalan, K.V.; Enemark, J.H.
    The pathogenic human sulfite oxidase mutants G473D and A208D are defective in intramolecular electron transfer (2005), Biochemistry, 44, 13734-13743.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.1 expressed in Escherichia coli strain TP1000 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.3.1 A208D the intramolecular electron transfer rate constants at pH 6.0 are decreased by 3 orders of magnitude relative to that of the wild type, the active site structure of the Mo(V) form of A208D is different from that of the wild type Homo sapiens
1.8.3.1 G473A dimer Homo sapiens
1.8.3.1 G473D monomer, the Mo(V) active site structure is similar to that of the wild type, and the IET rate constant is only 2.6fold smaller than that of the wild type Homo sapiens
1.8.3.1 G473D/R212A shows no intramolecular electron transfer rate Homo sapiens
1.8.3.1 G473W monomer Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.1 0.00129
-
 sulfite wild type enzyme in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 0.00453
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 0.00825
-
 sulfite wild type enzyme in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 0.0692
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 0.107
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 1.39
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 1.66
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 1.91
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 2.03
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 2.04
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 8.5 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.1 Superdex 200 FPLC column gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.1 sulfite + H2O + A
-
 Homo sapiens SO42- + AH2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.3.1 dimer wild type enzyme, mutant enzymes G473A and A208D Homo sapiens
1.8.3.1 monomer mutant enzymes G473D and G473W Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.3.1 SO
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.1 0.14
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 0.15
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 0.54
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 0.6
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 0.75
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 2.48
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 4.15
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 13.2
-
 sulfite wild type enzyme in 20 mM Tris at pH 6.0 Homo sapiens
1.8.3.1 26.9
-
 sulfite wild type enzyme in 20 mM Tris at pH 8.5 Homo sapiens
1.8.3.1 28.4
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 8.5 Homo sapiens