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Literature summary extracted from

  • Filipek, R.; Szczepanowski, R.; Sabat, A.; Potempa, J.; Bochtler, M.
    Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition (2004), Biochemistry, 43, 14306-14315.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.48 prostaphopain B, expression as GST-fusion protein in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.48 purified recombinant mature staphopain B, sitting drop vapour diffusion method at 4°C or 21°C, equal volume of protein solution containing 15 mg/ml protein in 5mM Tris, pH 7.5, and reservoir solution containing 50 mM Bis-Tris, pH 6.5, 20% w/v PEG 4000, or 100 mM HEPES, pH 7.5, 21% w/v PEG 4000, and 10% v/v 2-propanol, are mixed, a few weeks, X-ray diffraction structure determination and analysis at 2.5-2.8 A resolution Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.48 additional information the proregion of the zymogen is inhibitory for the mature enzyme Staphylococcus aureus
3.4.22.48 staphostatin
-
 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.48 Staphylococcus aureus
-
-
-
3.4.22.48 Staphylococcus aureus V8
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.48 additional information staphopain is synthesized as proenzyme, which is processed to the mature form by cleavage of the N-terminally propeptide, interactions between the propeptide and the mature enzyme, modeling, overview Staphylococcus aureus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.48 recombinant GST-fusion prostaphopain B from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, gel filtration, and cleavage of the GST-tag followed by ultrafiltration Staphylococcus aureus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.48 broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate active site and substrate binding subsite structures Staphylococcus aureus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.48 Bz-Pro-Phe-Arg-4-nitroanilide + H2O chromogenic substrate Staphylococcus aureus Bz-Pro-Phe-Arg + 4-nitroaniline
-
 ?
3.4.22.48 Bz-Pro-Phe-Arg-4-nitroanilide + H2O chromogenic substrate Staphylococcus aureus V8 Bz-Pro-Phe-Arg + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.48 More analysis of structure and fold of the proenzyme, including the propeptide, interactions between the propeptide and the mature enzyme, modeling, overview Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.48 staphopain B
-
 Staphylococcus aureus