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Literature summary extracted from

  • Xu, Y.; Mitra, B.
    A highly active, soluble mutant of the membrane-associated (S)-mandelate dehydrogenase from Pseudomonas putida (1999), Biochemistry, 38, 12367-12376.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.31 additional information MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach. This mutant is very similar to the wild-type membrane-bound enzyme in its spectroscopic properties, substrate specificity, catalytic activity, kinetic mechanism, and lack of reactivity toeards oxygen. It should prove to be a highly useful model for structural studies of MDH Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 0.085
-
 (S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.14
-
 Mandelate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.18
-
 (S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme Pseudomonas putida
1.1.99.31 0.24
-
 indoleglycolate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.29
-
 2-Hydroxyoctanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.49
-
 2-hydroxyisocaproate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.64
-
 3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.89
-
 2-hydroxyhexanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 1.3
-
 3-Phenyllactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 2.6
-
 2-Hydroxyisovalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 3.2
-
 2-hydroxyvalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 10.3
-
 2-Hydroxy-3-butenoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 13.2
-
 2-Hydroxybutyrate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 17.2
-
 2-Hydroxy-3-butynoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.31 membrane associated Pseudomonas putida 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 (R,S)-2-hydroxy-3-butenoic acid + acceptor
-
 Pseudomonas putida 2-oxo-3-butenoic acid + reduced acceptor
-
 ?
1.1.99.31 (R,S)-2-hydroxyisovalerate + acceptor
-
 Pseudomonas putida 2-oxo-isovalerate + reduced acceptor
-
 ?
1.1.99.31 (S)-mandelate + acceptor
-
 Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
 ?
1.1.99.31 2-hydroxy-3-butynoate + acceptor
-
 Pseudomonas putida 2-oxo-3-butynoate + reduced acceptor
-
 ?
1.1.99.31 2-hydroxybutyrate + acceptor
-
 Pseudomonas putida 2-oxobutyrate + reduced acceptor
-
 ?
1.1.99.31 2-hydroxyhexanoate + acceptor
-
 Pseudomonas putida 2-oxohexanoate + reduced acceptor
-
 ?
1.1.99.31 2-hydroxyisocaproate + acceptor
-
 Pseudomonas putida 2-oxoisocaproate + reduced acceptor
-
 ?
1.1.99.31 2-hydroxyoctanoate + acceptor
-
 Pseudomonas putida 2-oxooctanoate + reduced acceptor
-
 ?
1.1.99.31 2-hydroxyvalerate + acceptor
-
 Pseudomonas putida 2-oxovalerate + reduced acceptor
-
 ?
1.1.99.31 3-indolelactate + acceptor
-
 Pseudomonas putida 3-(1H-indol-2-yl)-2-oxopropanoate + reduced acceptor
-
 ?
1.1.99.31 3-phenyllactate + acceptor
-
 Pseudomonas putida 2-oxo-3-phenylpropanoate + reduced acceptor
-
 ?
1.1.99.31 4-chloromandelate + acceptor
-
 Pseudomonas putida 2-oxo-2-(4-chlorophenyl)acetate + reduced acceptor
-
 ?
1.1.99.31 indoleglycolate + acceptor
-
 Pseudomonas putida indol-2-yl(oxo)acetate + reduced acceptor
-
 ?
1.1.99.31 mandelate + acceptor
-
 Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.31 MDH
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.31 0.03
-
 2-Hydroxyisovalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.1
-
 2-Hydroxybutyrate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.48
-
 2-hydroxyhexanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.5
-
 2-hydroxyvalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.5
-
 2-Hydroxy-3-butenoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.87
-
 3-Phenyllactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 0.96
-
 2-Hydroxyoctanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 1.28
-
 2-hydroxyisocaproate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 3.13
-
 3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 3.85
-
 3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 6.6
-
 2-Hydroxy-3-butynoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 38.5
-
 indoleglycolate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 195
-
 Mandelate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 205
-
 (S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.1.99.31 290
-
 (S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme Pseudomonas putida