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Literature summary extracted from

  • Gomez-Garcia, M.R.; Losada, M.; Serrano, A.
    A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes (2006), Biochem. J., 395, 211-221.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 gene ppa1, isozyme PPase 1, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli Arabidopsis thaliana
3.6.1.1 gene ppa1, isozyme sPPase-I, DNA and amino acid sequence determination and analysis, phylogenetic analysis Chlamydomonas reinhardtii
3.6.1.1 gene ppa2, isozyme sPPase-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 Ca2+
-
 Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 chloroplast
-
 Arabidopsis thaliana 9507
-
3.6.1.1 chloroplast isozyme sPPase-I Chlamydomonas reinhardtii 9507
-
3.6.1.1 mitochondrion isozyme sPPase-II Chlamydomonas reinhardtii 5739
-
3.6.1.1 additional information isozyme subcellular localization study Chlamydomonas reinhardtii
-
-
3.6.1.1 soluble isozyme sPPase-I Chlamydomonas reinhardtii
-
-
3.6.1.1 soluble isozyme sPPase-II Chlamydomonas reinhardtii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Cu2+ can partially, upt to 30%, substitute for Mg2+ Chlamydomonas reinhardtii
3.6.1.1 Fe2+ can partially, upt to 30%, substitute for Mg2+ Chlamydomonas reinhardtii
3.6.1.1 Mg2+ required, best divalent cation Chlamydomonas reinhardtii
3.6.1.1 Mn2+ can partially, upt to 30%, substitute for Mg2+ Chlamydomonas reinhardtii
3.6.1.1 Zn2+ can partially, upt to 30%, substitute for Mg2+ Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 24000
-
 1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry Chlamydomonas reinhardtii
3.6.1.1 24350
-
 1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry Chlamydomonas reinhardtii
3.6.1.1 28000 32000 gel filtration and native PAGE Chlamydomonas reinhardtii
3.6.1.1 29850
-
 1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry Chlamydomonas reinhardtii
3.6.1.1 30000
-
 1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry Chlamydomonas reinhardtii
3.6.1.1 33000 37000 gel filtration and native PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Chlamydomonas reinhardtii
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Arabidopsis thaliana Q9LXC9 isozyme PPA1, chloroplast precursor; isozyme PPA1
-
3.6.1.1 Chlamydomonas reinhardtii Q93Y52 ppa1, chloroplast precursor; soluble inorganic pyrophosphatase isozyme I, plastidial isozyme sPPase-I, gene ppa1, is the major isozyme
-
3.6.1.1 Chlamydomonas reinhardtii Q949J1 ppa2; soluble inorganic pyrophosphatase isozyme II, minor isozyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.1 proteolytic modification the chloroplast isozyme I contains a precursor sequence, the recombinant enzyme is processed in bacterial cells Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 native isozyme sPPase-I by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration Chlamydomonas reinhardtii
3.6.1.1 native isozyme sPPase-II by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Chlamydomonas reinhardtii 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 monomer 1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry Chlamydomonas reinhardtii
3.6.1.1 monomer 1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry Chlamydomonas reinhardtii
3.6.1.1 More peptide mass fingerprint analysis of isozyme sPPase-I Chlamydomonas reinhardtii
3.6.1.1 More peptide mass fingerprint analysis of isozyme sPPase-II Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 chloroplast inorganic pyrophosphatase 1
-
 Arabidopsis thaliana
3.6.1.1 More the enzyme is a member of the sPPase family I Chlamydomonas reinhardtii
3.6.1.1 PPA1
-
 Arabidopsis thaliana
3.6.1.1 PPase 1
-
 Chlamydomonas reinhardtii
3.6.1.1 PPase 1
-
 Arabidopsis thaliana
3.6.1.1 PPase 2
-
 Chlamydomonas reinhardtii
3.6.1.1 pyrophosphate phospho-hydrolase 1
-
 Arabidopsis thaliana
3.6.1.1 soluble inorganic pyrophosphatase
-
 Chlamydomonas reinhardtii
3.6.1.1 soluble inorganic pyrophosphatase 2
-
 Chlamydomonas reinhardtii
3.6.1.1 sPPAse
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 22
-
 assay at room temperature Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 7.5
-
-
 Chlamydomonas reinhardtii