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Literature summary extracted from

  • Smekal, O.; Yasin, M.; Fewson, C.A.; Reid, G.A.; Chapman, S.K.
    L-mandelate dehydrogenase from Rhodotorula graminis: comparisons with the L-lactate dehydrogenase (flavocytochrome b2) from Saccharomyces cerevisiae (1993), Biochem. J., 290, 103-107.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.31 hexanoate
-
 Rhodotorula graminis
1.1.99.31 L-lactate
-
 Rhodotorula graminis
1.1.99.31 L-Phenyllactate
-
 Rhodotorula graminis
1.1.99.31 oxalate
-
 Rhodotorula graminis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 0.18
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: 2,6-dichloroindophenol Rhodotorula graminis
1.1.99.31 0.27
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: ferricyanide Rhodotorula graminis
1.1.99.31 0.49
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: cytochrome c Rhodotorula graminis

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Rhodotorula graminis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 (S)-mandelate + 2 ferricyanide
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+
-
 ?
1.1.99.31 (S)-mandelate + 2,6-dichloroindophenol
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + ?
-
 ?
1.1.99.31 (S)-mandelate + cytochrome c
-
 Rhodotorula graminis 2-oxo-2-phenylacetate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.31 L-mandelate dehydrogenase
-
 Rhodotorula graminis
1.1.99.31 L-MDH
-
 Rhodotorula graminis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.31 3 6 (S)-Mandelate pH 7.5, 25°C, electron acceptor: 2,6-dichloroindophenol Rhodotorula graminis
1.1.99.31 49
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: cytochrome c Rhodotorula graminis
1.1.99.31 109
-
 (S)-Mandelate pH 7.5, 25°C, electron acceptor: ferricyanide Rhodotorula graminis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.31 flavocytochrome b2 the enzyme is a flavocytochrome b2 Rhodotorula graminis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.99.31 0.078
-
 oxalate pH 7.5, 25°C Rhodotorula graminis
1.1.99.31 0.37
-
 hexanoate pH 7.5, 25°C Rhodotorula graminis
1.1.99.31 0.4
-
 L-lactate pH 7.5, 25°C Rhodotorula graminis
1.1.99.31 1.9
-
 L-Phenyllactate pH 7.5, 25°C Rhodotorula graminis