EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.5 | Ag+ | - |
Pseudomonas putida | |
3.5.5.5 | Co2+ | - |
Pseudomonas putida | |
3.5.5.5 | Cu2+ | - |
Pseudomonas putida | |
3.5.5.5 | Fe2+ | - |
Pseudomonas putida | |
3.5.5.5 | Hg2+ | - |
Pseudomonas putida | |
3.5.5.5 | Ni2+ | - |
Pseudomonas putida | |
3.5.5.5 | Zn2+ | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.5 | 3.61 | - |
phenyl acetonitrile | 35°C, pH 7.5 | Pseudomonas putida | |
3.5.5.5 | 5.31 | - |
indole 3-acetonitrile | 35°C, pH 7.5 | Pseudomonas putida | |
3.5.5.5 | 13.4 | - |
mandelonitrile | 35°C, pH 7.5 | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 43000 | - |
9 or 10 * 43000, SDS-PAGE | Pseudomonas putida |
3.5.5.5 | 412000 | - |
gel filtration | Pseudomonas putida |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.5.5.5 | dimethyl sulfoxide | 2.5 or 5%, up to 20% increase in activity, decrease above | Pseudomonas putida |
3.5.5.5 | Ethanol | 5%, 63% enhancement in activity probably due to increased availibaility of substrate. Above 10% ethanol, significant decrease in activity | Pseudomonas putida |
3.5.5.5 | Methanol | 5%, 78% enhancement in activity probably due to increased availibaility of substrate. Above 10% methanol, significant decrease in activity | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.5 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.5 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 3.26 | - |
35°C, pH 7.5 | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.5.5 | -80°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, 20% glycerol, stable | Pseudomonas putida |
3.5.5.5 | 4°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, rapid loss of activity, presence of 20% glycerol stabilizes | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.5 | 2-chlorophenyl acetonitrile + H2O | 76.1% of the activity with mandelonitrile | Pseudomonas putida | 2-chlorobenzoic acid + NH3 | - |
ir | |
3.5.5.5 | 2-methoxyphenyl acetonitrile + H2O | 44.5% of the activity with mandelonitrile | Pseudomonas putida | 2-methoxybenzoic acid + NH3 | - |
ir | |
3.5.5.5 | 2-thiophene acetonitrile + H2O | 137% of the activity with mandelonitrile | Pseudomonas putida | thiophene-2-carboxylic acid + NH3 | - |
ir | |
3.5.5.5 | 4-aminophenyl acetonitrile + H2O | 502.3% of the activity with mandelonitrile | Pseudomonas putida | 4-aminobenzoic acid + NH3 | - |
ir | |
3.5.5.5 | 4-chlorophenyl acetonitrile + H2O | 360.3% of the activity with mandelonitrile | Pseudomonas putida | 4-chlorobenzoic acid + NH3 | - |
ir | |
3.5.5.5 | 4-hydroxy benzonitrile + H2O | 23.1% of the activity with mandelonitrile | Pseudomonas putida | 4-hydroxybenzoate + NH3 | - |
ir | |
3.5.5.5 | 4-hydroxyphenyl acetonitrile + H2O | 435% of the activity with mandelonitrile | Pseudomonas putida | 4-hydroxybenzoic acid + NH3 | - |
ir | |
3.5.5.5 | adiponitrile + H2O | 17.2% of the activity with mandelonitrile | Pseudomonas putida | adipic acid + NH3 | - |
ir | |
3.5.5.5 | cyclohexane carbonitrile + H2O | 12.5% of the activity with mandelonitrile | Pseudomonas putida | cyclohexlycarboxylic acid + NH3 | - |
ir | |
3.5.5.5 | hex-5-enenitrile + H2O | 7.9% of the activity with mandelonitrile | Pseudomonas putida | hex-5-enoic acid + NH3 | - |
ir | |
3.5.5.5 | indole 3-acetonitrile + H2O | 319.9% of the activity with mandelonitrile | Pseudomonas putida | (indol-3-yl)acetic acid + NH3 | - |
ir | |
3.5.5.5 | isovaleronitrile + H2O | 11.4% of the activity with mandelonitrile | Pseudomonas putida | isovaleric acid + NH3 | - |
ir | |
3.5.5.5 | mandelonitrile + 2 H2O | - |
Pseudomonas putida | (R)-mandelic acid + NH3 | 99.9% enantiomeric excess for (R)-isomer | ir | |
3.5.5.5 | phenoxy acetonitrile + H2O | 232.6% of the activity with mandelonitrile | Pseudomonas putida | phenoxyacetate + NH3 | - |
ir | |
3.5.5.5 | phenyl acetonitrile + H2O | 342.3% of the activity with mandelonitrile | Pseudomonas putida | benzoic acid + NH3 | - |
ir | |
3.5.5.5 | phenyl glycinenitrile + H2O | 192.5% of the activity with mandelonitrile | Pseudomonas putida | phenylalanine + NH3 | - |
ir | |
3.5.5.5 | phenyl thioacetonitrile + H2O | 187.7% of the activity with mandelonitrile | Pseudomonas putida | thiophenylacetate + NH3 | - |
ir | |
3.5.5.5 | phenylacetonitrile + 2 H2O | - |
Pseudomonas putida | phenylacetate + NH3 | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.5.5 | decamer | 9 or 10 * 43000, SDS-PAGE | Pseudomonas putida |
3.5.5.5 | nonamer | 9 or 10 * 43000, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 40 | - |
- |
Pseudomonas putida |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 20 | - |
21% of maximum activity | Pseudomonas putida |
3.5.5.5 | 70 | - |
11% of maximum activity | Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 30 | - |
6 h, 87% residual activity | Pseudomonas putida |
3.5.5.5 | 40 | - |
half-life 76 min | Pseudomonas putida |
3.5.5.5 | 50 | - |
half-life 9 min | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.5 | 1.1 | - |
phenyl acetonitrile | 35°C, pH 7.5 | Pseudomonas putida | |
3.5.5.5 | 11.8 | - |
mandelonitrile | 35°C, pH 7.5 | Pseudomonas putida | |
3.5.5.5 | 13.4 | - |
indole 3-acetonitrile | 35°C, pH 7.5 | Pseudomonas putida | |
3.5.5.5 | 18.85 | - |
phenyl acetonitrile | 35°C, pH 7.5 | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 7 | - |
- |
Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 6.5 | - |
half-life 36 min | Pseudomonas putida |
3.5.5.5 | 7 | - |
half-life 110 min | Pseudomonas putida |
3.5.5.5 | 7.5 | - |
half-life 87 min | Pseudomonas putida |
3.5.5.5 | 8 | - |
half-life 53 min | Pseudomonas putida |