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Literature summary extracted from

  • Choi, Y.J.; Miguez, C.B.; Lee, B.H.
    Characterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96 (2004), Appl. Environ. Microbiol., 70, 3213-3221.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 2-mercaptoethanol
-
 Lacticaseibacillus casei
3.1.1.1 Ag+
-
 Lacticaseibacillus casei
3.1.1.1 DEPC histidine modifier Lacticaseibacillus casei
3.1.1.1 Hg2+
-
 Lacticaseibacillus casei
3.1.1.1 PAO cysteine modifier Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.014
-
 4-nitrophenyl caprylate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.019
-
 4-nitrophenyl caproate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.043
-
 4-nitrophenyl caprate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.097
-
 4-nitrophenyl butanoate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.134
-
 4-nitrophenyl acetate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.186
-
 4-nitrophenyl laurate pH 7.0, 37°C Lacticaseibacillus casei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 Ca2+ stimulation Lacticaseibacillus casei
3.1.1.1 Mn2+ stimulation Lacticaseibacillus casei
3.1.1.1 Zn2+ stimulation Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 67064
-
 x * 67500, SDS-PAGE, x * 67064, calculated, protein with S-tag Lacticaseibacillus casei
3.1.1.1 67500
-
 x * 67500, SDS-PAGE, x * 67064, calculated, protein with S-tag Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Lacticaseibacillus casei Q840T2
-
-
3.1.1.1 Lacticaseibacillus casei CL96 Q840T2
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.1 a carboxylic ester + H2O = an alcohol + a carboxylate catalytic triad consists of S325, D516, and H558 Lacticaseibacillus casei
a

Storage Stability

EC Number Storage Stability Organism
3.1.1.1 -20°C, 50 mM phosphate buffer, pH 7.0, 10% v/v glycerol, stable for several weeks Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Lacticaseibacillus casei 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Lacticaseibacillus casei CL96 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Lacticaseibacillus casei 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Lacticaseibacillus casei CL96 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl caprate + H2O
-
 Lacticaseibacillus casei 4-nitrophenol + capric acid
-
 ?
3.1.1.1 4-nitrophenyl caprate + H2O
-
 Lacticaseibacillus casei CL96 4-nitrophenol + capric acid
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O
-
 Lacticaseibacillus casei 4-nitrophenol + caproate
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O
-
 Lacticaseibacillus casei CL96 4-nitrophenol + caproate
-
 ?
3.1.1.1 4-nitrophenyl caprylate + H2O
-
 Lacticaseibacillus casei 4-nitrophenol + caprylate
-
 ?
3.1.1.1 4-nitrophenyl laurate + H2O
-
 Lacticaseibacillus casei 4-nitrophenol + lauric acid
-
 ?
3.1.1.1 4-nitrophenyl laurate + H2O
-
 Lacticaseibacillus casei CL96 4-nitrophenol + lauric acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 67500, SDS-PAGE, x * 67064, calculated, protein with S-tag Lacticaseibacillus casei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 37
-
-
 Lacticaseibacillus casei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 0.024
-
 4-nitrophenyl laurate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.032
-
 4-nitrophenyl acetate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.089
-
 4-nitrophenyl caprate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.099
-
 4-nitrophenyl butanoate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.119
-
 4-nitrophenyl caproate pH 7.0, 37°C Lacticaseibacillus casei
3.1.1.1 0.125
-
 4-nitrophenyl caprylate pH 7.0, 37°C Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7
-
-
 Lacticaseibacillus casei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 6 8 stable within this range Lacticaseibacillus casei