EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.2.2 | overexpression of His6-tagged DHP in Escherichia coli Novablue cells | Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.2 | 2.4 | - |
dihydrouracil | - |
Bacillus sp. (in: Bacteria) | |
3.5.2.2 | 5 | - |
hydantoin | - |
Bacillus sp. (in: Bacteria) |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.2 | Co2+ | high stimulation, stimulates enzyme activity most effectively | Bacillus sp. (in: Bacteria) | |
3.5.2.2 | Cu2+ | low stimulation | Bacillus sp. (in: Bacteria) | |
3.5.2.2 | Mn2+ | high stimulation | Bacillus sp. (in: Bacteria) | |
3.5.2.2 | additional information | no stimulation with Mg2+, Ni2+, Ca2+ and Fe2+ | Bacillus sp. (in: Bacteria) | |
3.5.2.2 | Zn2+ | low stimulation | Bacillus sp. (in: Bacteria) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.2.2 | 52000 | - |
SDS-PAGE corresponding well with calculated mass of the affinity-tagged translational product of the dhp gene | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.1 | Brevibacillus agri | - |
- |
- |
1.3.1.1 | Oryza sativa | Q6Z744 | - |
- |
1.3.1.1 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZNL7 | LT2 | - |
3.5.2.2 | Agrobacterium tumefaciens | Q44184 | - |
- |
3.5.2.2 | Bacillus sp. (in: Bacteria) | Q56S49 | TS-23 | - |
3.5.2.2 | Bacillus sp. (in: Bacteria) TS-23 | Q56S49 | TS-23 | - |
3.5.2.2 | Geobacillus stearothermophilus | Q45515 | - |
- |
3.5.2.2 | Pseudomonas putida | Q59699 | - |
- |
3.5.2.2 | Ralstonia pickettii | Q8VTT5 | - |
- |
3.5.2.2 | Streptomyces coelicolor | O69809 | - |
- |
3.5.2.2 | Thermus sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.2.2 | by immobilized metal affinity chromatography | Bacillus sp. (in: Bacteria) |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.2.2 | 0.17 | - |
EDTA-dialyzed/Co2+-substituted enzyme incubated with hydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.36 | - |
EDTA-dialyzed enzyme incubated with Fe2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.46 | - |
EDTA-dialyzed enzyme incubated with Ca2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.49 | - |
EDTA-dialyzed enzyme incubated with Ni2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.52 | - |
EDTA-dialyzed enzyme with D,L-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.54 | - |
EDTA-dialyzed enzyme incubated with Mg2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.62 | - |
EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-5(2-(methylthio)-ethyl)hydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.68 | - |
EDTA-dialyzed enzyme incubated with Cu2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 0.73 | - |
EDTA-dialyzed enzyme incubated with Zn2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 1.69 | - |
EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 2.38 | - |
EDTA-dialyzed enzyme incubated with Mn2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 3.56 | - |
EDTA-dialyzed/Co2+-substituted enzyme incubated with dihydrouracil as substrate | Bacillus sp. (in: Bacteria) |
3.5.2.2 | 3.6 | - |
EDTA-dialyzed enzyme incubated with Co2+ with DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.2.2 | 50°C, 10 days | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.2 | 5,6-dihydrouracil + H2O | highest activity of the EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.5.2.2 | 5,6-dihydrouracil + H2O | highest activity of the EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) TS-23 | ? | - |
? | |
3.5.2.2 | DL-5(2-(methylthio)-ethyl)hydantoin + H2O | EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.5.2.2 | DL-5(2-(methylthio)-ethyl)hydantoin + H2O | EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) TS-23 | ? | - |
? | |
3.5.2.2 | DL-p-hydroxyphenylhydantoin + H2O | EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.5.2.2 | DL-p-hydroxyphenylhydantoin + H2O | EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) TS-23 | ? | - |
? | |
3.5.2.2 | hydantoin + H2O | lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.5.2.2 | hydantoin + H2O | lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme | Bacillus sp. (in: Bacteria) TS-23 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.2.2 | DHP | - |
Thermus sp. |
3.5.2.2 | DHP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
3.5.2.2 | DHP | - |
Brevibacillus agri |
3.5.2.2 | DHP | - |
Bacillus sp. (in: Bacteria) |
3.5.2.2 | DHP | - |
Geobacillus stearothermophilus |
3.5.2.2 | DHP | - |
Pseudomonas putida |
3.5.2.2 | DHP | - |
Ralstonia pickettii |
3.5.2.2 | DHP | - |
Agrobacterium tumefaciens |
3.5.2.2 | DHP | - |
Streptomyces coelicolor |
3.5.2.2 | DHP | - |
Oryza sativa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.2.2 | 60 | - |
DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.2.2 | 50 | 80 | DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.2 | 3.04 | - |
hydantoin | - |
Bacillus sp. (in: Bacteria) | |
3.5.2.2 | 6.2 | - |
dihydrouracil | - |
Bacillus sp. (in: Bacteria) |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.2.2 | 8 | - |
DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.2.2 | 7 | 9 | DL-p-hydroxyphenylhydantoin as substrate | Bacillus sp. (in: Bacteria) |