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Literature summary extracted from

  • Assa, P.; Ozkan, M.; Ozcengiz, G.
    Thermostability and regulation of Clostridium thermocellum L-lactate dehydrogenase expressed in Escherichia coli (2005), Ann. Microbiol., 55, 193-197.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 expression in Escherichia coli Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 ATP 10 mM, 40% loss of activity Acetivibrio thermocellus
1.1.1.27 D-fructose-1,6-diphosphate 5 mM, 25% loss of activity Acetivibrio thermocellus
1.1.1.27 NaCl 2 M, 36% loss of activity Acetivibrio thermocellus
1.1.1.27 NAD+ 40 mM, complete loss of activity Acetivibrio thermocellus
1.1.1.27 oxalate 0.5 mM, 28% inhibition Acetivibrio thermocellus
1.1.1.27 oxamate 0.5 mM, 41% inhibition Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.27 Mg2+ 20-30 mM, 10% increase in activity Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Acetivibrio thermocellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 partial Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 50
-
-
 Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 additional information
-
 very stable at room temperature, 4°C and 50°C Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 7.5
-
-
 Acetivibrio thermocellus