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Literature summary extracted from

  • Biswal, B.K.; Au, K.; Cherney, M.M.; Garen, C.; James, M.N.
    The molecular structure of Rv2074, a probable pyridoxine 5'-phosphate oxidase from Mycobacterium tuberculosis, at 1.6 angstroms resolution (2006), Acta Crystallogr. Sect. F, 62, 735-742.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.5 expression of GST-tagged wild-type and of GST-tagged selenomethionine-labeled enzymes from strain Rv2074 in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.5 purified recombinant enzyme from strain Rv2074, sitting drop vapour diffusion method in microtiter plates at room temperature, 12 mg/ml enzyme in 50 mM Tris-HCl, pH 8.0, 5 mM DTT, and glutathione, mixed with an equal volume of precipitation solution containing 0.2 M sodium citrate, pH 5.0, 30% glycerol, and 20% PEG 4000, hanging drop vapour diffusion with 0.5 ml protein and 1 ml precipitation solutions mixed, 1-2 weeks, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.0 A, modeling Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.5 15000
-
 x * 15000, recombinant detagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Mycobacterium tuberculosis
-
-
-
1.4.3.5 Mycobacterium tuberculosis Rv2074
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 recombinant GST-tagged wild-type and of GST-tagged selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, removal of the GST-tags by rTEV protease, followed by dialysis and ultrafiltration Mycobacterium tuberculosis

Subunits

EC Number Subunits Comment Organism
1.4.3.5 ? x * 15000, recombinant detagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 PNPOx
-
 Mycobacterium tuberculosis
1.4.3.5 pyridoxine 5'-phosphate oxidase
-
 Mycobacterium tuberculosis