BRENDA - Enzyme Database

Overexpression, crystallization and preliminary X-ray crystallographic analysis of erythronate-4-phosphate dehydrogenase from Pseudomonas aeruginosa

Ha, J.Y.; Lee, J.H.; Kim, K.H.; Kim, D.J.; Lee, H.H.; Kim, H.K.; Yoon, H.J.; Suh, S.W.; Acta Crystallogr. Sect. F 62, 139-141 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.290
expressed in Escherichia coli
Pseudomonas aeruginosa
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.290
hanging-drop vapour diffusion at 24°C K using 0.7 M ammonium dihydrogen phosphate, 0.4 M ammonium tartrate, 0.1 M sodium citrate pH 5.6 and 10 mM cupric chloride
Pseudomonas aeruginosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.290
83000
-
estimated by dynamic light-scattering analysis
Pseudomonas aeruginosa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.290
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.290
metal-chelate chromatography on Ni-NTA resin and size exclusion chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.290
erythronate-4-phosphate + NAD+
-
667077
Pseudomonas aeruginosa
3-hydroxy-4-phospho-hydroxy-alpha-ketobutyrate + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.290
homodimer
2 * 42 067, enzyme including a C-terminal tag, enzyme consists of two identical 380-residue subunits
Pseudomonas aeruginosa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.290
NAD+
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.290
expressed in Escherichia coli
Pseudomonas aeruginosa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.290
NAD+
-
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.290
hanging-drop vapour diffusion at 24°C K using 0.7 M ammonium dihydrogen phosphate, 0.4 M ammonium tartrate, 0.1 M sodium citrate pH 5.6 and 10 mM cupric chloride
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.290
83000
-
estimated by dynamic light-scattering analysis
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.290
metal-chelate chromatography on Ni-NTA resin and size exclusion chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.290
erythronate-4-phosphate + NAD+
-
667077
Pseudomonas aeruginosa
3-hydroxy-4-phospho-hydroxy-alpha-ketobutyrate + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.290
homodimer
2 * 42 067, enzyme including a C-terminal tag, enzyme consists of two identical 380-residue subunits
Pseudomonas aeruginosa