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Literature summary extracted from

  • Ohki, T.; Mizuno, N.; Shibata, N.; Takeo, M.; Negoro, S.; Higuchi, Y.
    Crystallization and X-ray diffraction analysis of 6-aminohexanoate-dimer hydrolase from Arthrobacter sp. KI72 (2005), Acta Crystallogr. Sect. F, 61, 928-930.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.46 nylB24 gene, expression of the His-tagged EII-EII'-hybrid Hyb24 in Escherichia coli strain KP3998 Arthrobacter sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.46 purified recombinant Hyb24, sitting drop vapor diffusion, 38 mg/ml protein in 20 mM phosphate, pH 7.3, and 10% glycerol, 0.02 ml of protein solution is mixed with the same volume of reservoir solution and equilibrated against 0.8 ml of reservoir solution, 10°C, 48 h, preparation of methylmercuric chloride heavy atom derivatives, X-ray diffraction structure determination and analysis at 1.75-1.8 A resolution Arthrobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.46 T3A/P4R/T5S/S8Q/D15G construction of a hybrid of isozymes EII and EII', termed Hyb24, by five amino acid replacement in EII', the mutant shows the same activity as EII' Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O Arthrobacter sp. the enzyme is responsible for the degradation of nylon-6 industrial production by-products 6-aminohexanoate
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?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.46 Arthrobacter sp.
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formerly Flavobacterium sp., strain K172, enzyme form EII and cryptic enzyme form EII'
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.46 recombinant His-tagged EII-EII'-hybrid Hyb24 from Escherichia coli strain KP3998 by anion exchange chromatography, gel filtration, and again anion exchange chromatography, to homogeneity Arthrobacter sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O = 2 6-aminohexanoate structure-function relationship Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O the enzyme is responsible for the degradation of nylon-6 industrial production by-products Arthrobacter sp. 6-aminohexanoate
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?
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O the enzyme hydrolyzes the linear dimer substrate Arthrobacter sp. 6-aminohexanoate
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?