Literature summary extracted from
Ohki, T.; Mizuno, N.; Shibata, N.; Takeo, M.; Negoro, S.; Higuchi, Y.
Crystallization and X-ray diffraction analysis of 6-aminohexanoate-dimer hydrolase from Arthrobacter sp. KI72 (2005), Acta Crystallogr. Sect. F, 61, 928-930.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.1.46 |
nylB24 gene, expression of the His-tagged EII-EII'-hybrid Hyb24 in Escherichia coli strain KP3998 |
Arthrobacter sp. |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.5.1.46 |
purified recombinant Hyb24, sitting drop vapor diffusion, 38 mg/ml protein in 20 mM phosphate, pH 7.3, and 10% glycerol, 0.02 ml of protein solution is mixed with the same volume of reservoir solution and equilibrated against 0.8 ml of reservoir solution, 10°C, 48 h, preparation of methylmercuric chloride heavy atom derivatives, X-ray diffraction structure determination and analysis at 1.75-1.8 A resolution |
Arthrobacter sp. |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.5.1.46 |
T3A/P4R/T5S/S8Q/D15G |
construction of a hybrid of isozymes EII and EII', termed Hyb24, by five amino acid replacement in EII', the mutant shows the same activity as EII' |
Arthrobacter sp. |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.5.1.46 |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O |
Arthrobacter sp. |
the enzyme is responsible for the degradation of nylon-6 industrial production by-products |
6-aminohexanoate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.1.46 |
Arthrobacter sp. |
- |
formerly Flavobacterium sp., strain K172, enzyme form EII and cryptic enzyme form EII' |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.5.1.46 |
recombinant His-tagged EII-EII'-hybrid Hyb24 from Escherichia coli strain KP3998 by anion exchange chromatography, gel filtration, and again anion exchange chromatography, to homogeneity |
Arthrobacter sp. |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.5.1.46 |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O = 2 6-aminohexanoate |
structure-function relationship |
Arthrobacter sp. |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.1.46 |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O |
the enzyme is responsible for the degradation of nylon-6 industrial production by-products |
Arthrobacter sp. |
6-aminohexanoate |
- |
? |
|
3.5.1.46 |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O |
the enzyme hydrolyzes the linear dimer substrate |
Arthrobacter sp. |
6-aminohexanoate |
- |
? |
|